A new approach for improving cordycepin productivity in surface liquid culture of Cordyceps militaris using high-energy ion beam irradiation
A new approach for improving cordycepin productivity in surface liquid culture of Cordyceps militaris using high-energy ion beam irradiation
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DOI：
10.1111/j.1472-765x.2008.02456.x
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发表时间：
2008-12-01
影响因子：
2.4
通讯作者：
Sakakibara, M.
中科院分区：
文献类型：
--
作者：
Das, S. K.;Masuda, M.;Sakakibara, M.
利用离子束诱变技术获得蛹虫草突变株，并对其进行虫草素的高产研究。通过高能质子束对蛹虫草NBRC 9787进行诱变，在突变体筛选中获得了30类8-氮杂腺嘌呤抗性突变体和28类8-氮杂鸟嘌呤抗性突变体，其中7类被选为具有抗菌能力作为虫草素生产指标的有希望的初步突变体。在表面液体培养技术中，一些8-氮杂腺嘌呤抗性突变体表现出更好的虫草素生产性能;相比之下，在8-氮杂鸟嘌呤抗性突变体中，显示突变体编号G81-3在葡萄糖代谢速率和虫草素生产率方面远远优于对照。在初步优化使用的丰富的培养基，虫草素的生产是3.1和1.8克升（-1）的21天的培养突变体编号。G81-3和对照，分别。该突变株的虫草素产量比对照提高了72%，质子束诱变获得的突变株虫草素产量比对照高，诱变获得的突变株虫草素产量比对照高，具有优良的上级生产性能，可应用于工业生物技术领域，实现虫草素的大规模生产。
To obtain a higher cordycepin production using Cordyceps militaris mutant obtained by a new mutagenesis technique called 'ion beam'.Successful irradiation of C. militaris NBRC 9787 by a proton beam with high energy was performed, and 30 classes of 8-azaadenine- and 28 classes of 8-azaaguanine-resistant mutants were obtained on mutant screening, of which seven classes were selected as promising preliminary mutants having an antibacterial ability as an index of cordycepin production. In a surface liquid culture technique, some of the 8-azaadenine-resistant mutants gave a better performance for the cordycepin productivity; in contrast, among the 8-azaaguanine-resistant mutants, it was shown that mutant no. G81-3 was much better than the control in the metabolic rate of glucose and the cordycepin productivity. In primary optimization using the enriched medium, the cordycepin production was 3.1 and 1.8 g l(-1) on 21-day culture for mutant no. G81-3 and the control, respectively. The cordycepin production obtained by the mutant was 72% more than the control.The mutant obtained by proton beam irradiation had higher productivity of cordycepin than that of the control.The mutant obtained by irradiation had a superior production performance of cordycepin, and therefore, it could be used in the realm of applied industrial biotechnology for the large-scale production of cordycepin.