P-3A AND (-)-DESACETAMIDO P-3A - DEMONSTRATION AND STUDY OF THEIR EFFECTIVE FUNCTIONAL CLEAVAGE OF DUPLEX DNA
P-3A AND (-)-DESACETAMIDO P-3A - DEMONSTRATION AND STUDY OF THEIR EFFECTIVE FUNCTIONAL CLEAVAGE OF DUPLEX DNA
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DOI：
10.1016/s0960-894x(00)80449-7
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发表时间：
1992-12-01
影响因子：
2.7
通讯作者：
YANG, WJ
中科院分区：
文献类型：
--
作者：
BOGER, DL;YANG, WJ
研究了P-3A(1)和(-)-去乙酰氨基P-3A(2)的Fe(II)络合物对双链DNA的切割能力，通过检测超螺旋phiX 174 RFI DNA在O2存在下的单链和双链切割分别产生松弛(II)和线形(III)DNA。与Fe(II)-博莱霉素A2和去糖博莱霉素A2一样，Fe(II)-1和Fe(II)-2能有效地切割超螺旋phiX174 DNA。与Fe(II)-博莱霉素A2或脱糖博莱霉素A2不同，Fe(II)-L和2选择性地切割W794DNA，没有可识别的序列，这表明包括联噻唑在内的博莱霉素C端四肽S亚基的多核苷酸识别可能主导了博莱霉素A2DNA的切割选择性。
A study of the Fe(II) complexes of P-3A (1) and (-)-desacetamido P-3A (2) abilities to cleave duplex DNA was conducted through examination of single-strand and double-strand cleavage of supercoiled phiX 174 RFI DNA (Form I) in the presence of O2 to produce relaxed (Form II) and linear (Form III) DNA, respectively. Like Fe(II)-bleomycin A2 and deglycobleomycin A2, Fe(II)-1 and 2 effectively produced both single- and double-strand cleavage of supercoiled phiX174 DNA. Unlike Fe(II)-bleomycin A2 or deglycobleomycin A2, Fe(II)-l and 2 were found to cleave duplex w794 DNA with no discernible sequence selectively suggesting that the polynucleotide recognition of the C-terminus tetrapeptide S subunit of the bleomycins including the bithiazole may dominate the bleomycin A2 DNA cleavage selectivity.