Super-resolution spinning-disk confocal microscopy using optical photon reassignment
Super-resolution spinning-disk confocal microscopy using optical photon reassignment
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DOI：
10.1364/oe.23.015003
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发表时间：
2015-06-01
期刊：
影响因子：
3.8
通讯作者：
Kei, Takayuki
中科院分区：
文献类型：
--
作者：
Azuma, Takuya;Kei, Takayuki
旋转圆盘共聚焦显微镜是一种经过验证的技术，用于研究生物样品的3D结构。在这里，我们报告了一个超分辨率方法的基础上旋转盘共聚焦显微镜，光学提高横向分辨率的1.37倍，一次曝光。此外，反卷积产生两倍的衍射极限以上的改善。在新修改的Nipkow盘的帮助下，该盘分别在正面和背面包括针孔和微透镜，从样品发射的光子可以被光学地重新分配到它们起源的最可能的位置。因此，实现了分辨率的提高，保留了共焦显微镜固有的切片能力。这种极其简单的实现将使生物医学常规研究中的超高分辨率的可靠观测成为可能。(C)2015年美国光学学会
Spinning-disk confocal microscopy is a proven technology for investigating 3D structures of biological specimens. Here we report a super-resolution method based on spinning-disk confocal microscopy that optically improves lateral resolution by a factor of 1.37 with a single exposure. Moreover, deconvolution yields twofold improvement over the diffraction limit. With the help of newly modified Nipkow disk which comprises pinholes and micro-lenses on the front and back respectively, emitted photons from specimen can be optically reassigned to the most probable locations they originate from. Consequently, the improvement in resolution is achieved preserving inherent sectioning capabilities of confocal microscopy. This extremely simple implementation will enable reliable observations at super high resolution in biomedical routine research. (C) 2015 Optical Society of America