EXPRESSION OF EGF, EGF-RECEPTOR, P53, V-ERB-B AND RAS P21 IN COLORECTAL NEOPLASMS BY IMMUNOSTAINING PARAFFIN-EMBEDDED TISSUES
EXPRESSION OF EGF, EGF-RECEPTOR, P53, V-ERB-B AND RAS P21 IN COLORECTAL NEOPLASMS BY IMMUNOSTAINING PARAFFIN-EMBEDDED TISSUES
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DOI：
10.1111/j.1440-1827.1994.tb01696.x
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发表时间：
1994-02-01
影响因子：
2.2
通讯作者：
ITOH, H
中科院分区：
文献类型：
--
作者：
HAYASHI, Y;WIDJONO, YW;ITOH, H
通过免疫组化研究阐明表皮生长因子（EGF）、EGF受体（EGFR）、p53、v-erb B、ras p21在23例管状腺瘤和腺癌中表达或过表达的意义。分析 46 例结直肠肿瘤石蜡包埋组织中 EGF、EGFR、p53、v-erb B、ras p21 的表达情况（腺瘤：23 例；轻度不典型增生 14 例，中度不典型增生 6 例，重度不典型增生 3 例；腺癌：23 例；高分化 17 例，中分化 2 例，低分化 3 例，粘液癌 1 例）使用抗 EGF、EGFR、p53、v-erb B 和 ras p21 抗体进行免疫组织化学检测，EGF 和 ras p21 在癌病例中的表达比在腺瘤病例中更强，在重度和中度不典型增生中的表达比在轻度不典型增生中更强（EGF：在 5 个腺瘤 [21.74%] 和 17 个腺癌 [73.91%] 中染色呈阳性；ras p21： 6 例腺瘤（26.09%）和 14 例腺癌（60.87%）中 EGFR 染色呈阳性，2 例（8.70%）和 2 例腺癌（8.70%）p53 和 v-erb B 仅在癌病例中呈阳性（p53：4 例（17.39%）呈阳性；v-erb B：8 例呈阳性。例[34.78%]）。这项研究表明，这些因素似乎在结肠肿瘤的进展中发挥了一定作用，这表明基因改变并不总是等于蛋白质产物的过度表达，但它很好地反映了它们，并且染色对结直肠肿瘤的鉴别诊断做出了一些贡献。
Immunohistochemical studies were performed to clarify the significance of the expression or overexpression of epidermal growth factor (EGF), EGF-receptor (EGFR), p53, v-erb B, ras p21 in 23 cases each of tubular adenoma and adenocarcinoma. The expression of EGF, EGFR, p53, v-erb B, and ras p21 in paraffin-embedded tissues, from 46 patients with colorectal tumors (adenoma: 23 cases; 14 mild dysplasia, six moderate dysplasia, three severe dysplasia, adenocarcinoma: 23 cases; 17 well differentiated, two moderately differentiated, three poorly differentiated, one mucinous carcinoma was analyzed immunohistochemially using anti-EGF, EGFR, p53, v-erb B and ras p21 antibodies. The EGF and ras p21 tended to express more strongly in carcinoma cases than in the adenoma cases, and in severe and moderate dysplasia than in mild dysplasia (EGF: stained positive in five adenomas [21.74%] and 17 adenocarcinomas [73.91%]; ras p21: stained positive in six adenomas [26.09%] and 14 adenocarcinomas [60.87%]. The EGFR stained positive in two adenomas (8.70%) and two adenocarcinomas (8.70%). The p53 and v-erb B showed positive staining only in the carcinoma cases (p53: stained positive in four cases [17.39%]; v-erb B: stained positive in eight cases [34.78%]). This study suggests that these factors seem to have some role in the progression of colon neoplasms. It suggests that genetic alteration is not always equal to the overexpression of protein products, but that it reflects them well, and that the staining makes some contribution to differential diagnosis in colorectal neoplasms.