Facile synthesis of folate-conjugated magnetic/fluorescent bifunctional microspheres.
Facile synthesis of folate-conjugated magnetic/fluorescent bifunctional microspheres.
复制标题
叶酸共轭磁性/荧光双功能微球的简便合成
DOI：
10.1186/1556-276x-9-558
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发表时间：
2014
影响因子：
--
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Shen M;Jia W;Lin C;Fan G;Jin Y;Chen X;Chen G
本文研究了由叶酸功能化的二氧化硅壳层中的CdTe量子点和磁性核组成的磁性微球的功能成像性能。采用溶剂热法制备壳聚糖包覆的Fe 3 O 4纳米粒子（CS-coated Fe 3 O 4 NPs），在NHS和EDC的活化下，通过羧基和氨基的反应得到CdTe-CS包覆的Fe 3 O 4 NPs，最后通过Stöber法生长SiO2壳层，并通过光致发光（PL）猝灭（Fe 3 O 4-CdTe@SiO2）。此外，为了具有特异的靶向能力，通过酰胺化反应将FA分子与SiO2壳层键合，制备了磁性和荧光双功能微球（Fe 3 O 4-CdTe@SiO2-FA）。采用紫外-可见光谱、荧光光谱、傅里叶变换红外光谱、X射线粉末衍射、扫描透射电镜、透射电镜、热重分析和振动样品磁强计对复合纳米粒子的形貌、尺寸、化学成分和磁性进行了表征。结果表明，磁性荧光双功能微球具有较强的发光性，可用于HeLa细胞的免疫标记和荧光成像。
In this paper, we investigated the functional imaging properties of magnetic microspheres composed of magnetic core and CdTe quantum dots in the silica shell functionalized with folic acid (FA). The preparation procedure included the preparation of chitosan-coated Fe3O4nanoparticles (CS-coated Fe3O4NPs) prepared by a one-pot solvothermal method, the reaction between carboxylic and amino groups under activation of NHS and EDC in order to obtain the CdTe-CS-coated Fe3O4NPs, and finally the growth of SiO2shell vent the photoluminescence (PL) quenching via a Stöber method (Fe3O4-CdTe@SiO2). Moreover, in order to have a specific targeting capacity, the magnetic and fluorescent bifunctional microspheres were synthesized by bonding of SiO2shell with FA molecules via amide reaction (Fe3O4-CdTe@SiO2-FA). The morphology, size, chemical components, and magnetic property of as-prepared composite nanoparticles were characterized by ultraviolet-visible spectroscopy, fluorescent spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), scanning transmission electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM), respectively. The results show that the magnetic and fluorescent bifunctional microspheres have strong luminescent which will be employed for immuno-labeling and fluorescent imaging of HeLa cells.