Quantifying Changes in Chromosome Position to Assess Chromokinesin Activity.
Quantifying Changes in Chromosome Position to Assess Chromokinesin Activity.
复制标题
量化染色体位置的变化以评估染色因子活性。
DOI：
10.1007/978-1-0716-1904-9_10
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Stumpff,Jason
中科院分区：
文献类型：
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作者：
Thompson,AlexF;Vandal,Sarah;Stumpff,Jason
The chromokinesin KIF22 (Kid, kinesin-10 family) is the primary generator of polar ejection forces, which contribute to chromosome positioning and alignment in mitotic cells. Assessment of KIF22 function requires quantitative comparison of relative polar ejection forces between experimental conditions. This is facilitated by the generation of monopolar spindles to reduce the impact of bioriented microtubule attachment at kinetochores on chromosome positions and increase the dependence of chromosome positions on chromokinesin activity. Radial profile plots measure the intensity of chromatin signal in concentric circles around the poles of monopolar cells and represent an expedient quantitative measure of relative polar ejection forces. As such, this assay can be used to measure changes in polar ejection forces resulting from chromokinesin depletion or perturbation.
