HYDRODYNAMIC ALTERATIONS DURING DISTAL TUBULAR FLUID COLLECTIONS IN RAT-KIDNEY
HYDRODYNAMIC ALTERATIONS DURING DISTAL TUBULAR FLUID COLLECTIONS IN RAT-KIDNEY
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DOI：
10.1152/ajprenal.1977.232.6.f497
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发表时间：
1977-01-01
影响因子：
--
通讯作者：
GOTTSCHALK, CW
中科院分区：
文献类型：
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作者：
COLINDRES, RE;KRAMP, RA;GOTTSCHALK, CW
方法雄性Wistar大鼠55只，体质量200~310g，由Hilltop实验室动物公司提供，斯科茨代尔，Penna。动物禁食过夜，以50 mg/kg体重的戊巴比妥钠腹腔注射麻醉。在实验过程中，根据需要给予小剂量间断静脉注射麻醉剂。静脉输液使用颈静脉，颈动脉用于采血，在某些情况下，使用P23Db Statham压力传感器(Statham Instruments，Oxnard，加利福尼亚州)测量血压。连接到Beckman动态记录仪(Beckman Instruments，加利福尼亚州富勒顿)这些动物被放在加热的动物桌子上，体温保持在37摄氏度恒定。如前所述，打开腹腔，左肾准备进行显微穿刺术(12)。覆盖肾脏的腹膜反射和肾周脂肪完好无损。根据动物的利尿情况，使用PE-10或PE-50聚乙烯导管(Clay Adams，Parsippany，NJ)从双侧输尿管收集尿液。只有那些近端晚期的动物
METHODS Experiments were performed, except when otherwise stated, on 55 male Wistar rats, weighing 200-310 g, supplied by Hilltop Laboratory Animals, Scottsdale, Penna. The animals were fasted overnight and anesthetized with intraperitoneal sodium pentobarbital, 50 mg/kg body wt. During the experiment, small intermittent, intravenous doses of the anesthetic were given as needed. A jugular vein was used for the intravenous infusions and a carotid artery was used for blood sampling and, in some instances, for blood pressure measurements with a P23Db Statham pressure transducer (Statham Instruments, Oxnard, Calif.) connected to a Beckman dynograph recorder (Beckman Instruments, Fullerton, Calif.) The animals were placed on a heated animal table and the body temperature was maintained constant at 37OC. The abdominal cavity was opened and the left kidney was prepared for micropuncture as previously described (12). The peritoneal reflection covering the kidney and the perirenal fat were left intact. Urine was collected from both ureters with the use of a PE-10 or PE-50 polyethylene catheter (Clay Adams, Parsippany, NJ), depending on the diuretic condition of the animals. Only those animals having a late proximal