Characterization of the branched-photocycle intermediates P and Q of bacteriorhodopsin
Characterization of the branched-photocycle intermediates P and Q of bacteriorhodopsin
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DOI：
10.1021/jp021221p
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发表时间：
2002-12-26
影响因子：
3.3
通讯作者：
Birge, RR
中科院分区：
文献类型：
--
作者：
Gillespie, NB;Wise, KJ;Birge, RR
对细菌视紫红质分支光环中间体P和Q的生色团异构体含量、光化学来源、动力学异质性和光可逆性进行了研究。将这些蓝移的物种与通过热变性产生的具有相似光谱性质的产品进行比较。我们观察到，热化学和光化学物种在异构体含量和结合位置环境上都不同。含低浓度水的细菌视紫红质甘油悬浮液的顺序双光子激活(
The bacteriorhodopsin branched-photocycle intermediates P and Q are studied with respect to chromophore isomeric content, photochemical origin, kinetic heterogeneity, and photoreversibility. These blue-shifted species are compared to products with similar spectroscopic properties generated via thermal denaturation. We observe that the thermal and photochemical species differ in both isomeric content and binding site environment. Sequential two-photon activation of glycerol suspensions of bacteriorhodopsin containing low concentrations of water (