Androgen Receptor Binding to an Androgen-Responsive Element in the Promoter of the Srsf4 Gene Inhibits its Expression in Mouse Sertoli Cells
Androgen Receptor Binding to an Androgen-Responsive Element in the Promoter of the Srsf4 Gene Inhibits its Expression in Mouse Sertoli Cells
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DOI：
10.1002/mrd.22576
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发表时间：
2015-12-01
影响因子：
2.5
通讯作者：
Gui, Yaoting
中科院分区：
文献类型：
--
作者：
Guo, Huan;Li, Yuchi;Gui, Yaoting
The serine/arginine-rich splicing actor 4 (SRSF4) is essential for pre-mRNA splicing and can influence alternative-splice-site choice. Little is known about the specific function of this gene in the reproductive system, although a recent study identified a SRSF4 polymorphism significantly associated with a decreased risk of non-obstructive azoospermia in Chinese men. We previously found that the expression of Srsf4 was up-regulated in the testes of Sertoli-cell-selective androgen receptor knockout (S-Ar-/y) mice compared to wild-type mice using digital gene expression analysis. In this study, we confirmed and extended the selective gene expression data: SRSF4 was mainly located in the nucleus of Sertoli cells, and Srsf4 expression in the Sertoli-cell-derived cell line TM4 is down-regulation by testosterone. Moreover, androgen receptor directly binds the androgen-responsive element of the Srsf4 promoter. Taken together, these results demonstrate that Srsf4 is a direct downstream target of the androgen receptor in mouse Sertoli cells. (C) 2015 Wiley Periodicals, Inc.
