Mitochondrial fission is necessary for mitophagy, development and virulence of the insect pathogenic fungusBeauveria bassiana
Mitochondrial fission is necessary for mitophagy, development and virulence of the insect pathogenic fungusBeauveria bassiana
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线粒体裂变对于昆虫病原真菌白僵菌的线粒体自噬、发育和毒力是必需的
DOI：
10.1111/jam.14619
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发表时间：
2020-08-01
影响因子：
4
通讯作者：
Ying, S-H
中科院分区：
文献类型：
--
作者：
Wang, J-J;Peng, Y-J;Ying, S-H
目的线粒体分裂是维持线粒体形态和功能的基本动力。本研究试图确定线粒体分裂在丝状昆虫病原真菌球孢白僵菌中的作用。材料与方法通过蛋白质胞内定位和基因中断突变体的构建，对3个裂变相关基因(BbFis1、BbMdv1和BbDnm1)进行功能鉴定。结果只观察到与BbMdv1相互作用的BbFis1的线粒体定位，而BbMdv1不与BbDnm1相互作用。三个基因的单一中断突变产生了拉长和增大的线粒体，这些线粒体不能通过有丝分裂消除。与野生型相比，3株突变型菌株的ATP合成和营养生长均降低。3个基因在分生孢子形成的早期阶段参与，而在分生孢子形成的后期不参与。在淹水条件下，三个基因均对孢子发育有显著的促进作用，其中BbMdv1基因的缺失影响最大，而BbFis1和BbDnm1基因的缺失影响最小。最后，三个基因的中断显著降低了真菌的毒力，但它们的突变有不同的影响。结论除了在线粒体分裂和有丝分裂中的一致作用外，三个与分裂相关的基因在昆虫病原真菌B的发育和毒力中发挥着不同的作用。球孢菌。本研究的意义和影响本研究表明线粒体分裂与B细胞的生命周期有关。球孢菌。这些发现为真菌生理学的操作提供了信息，并促进了昆虫病原真菌的应用。
Aims Mitochondrial fission is an essential dynamics that maintains mitochondrial morphology and function. This study seeks to determine the roles of mitochondrial fission in the filamentous entomopathogenic fungusBeauveria bassiana. Material and methods Three fission-related genes (BbFis1,BbMdv1andBbDnm1) were functionally characterized via protein intracellular localization and construction of gene disruption mutants. Results Mitochondrial localization was only observed for BbFis1 which interacts with BbMdv1, but BbMdv1 did not have interaction with BbDnm1. Single disruption mutant of three genes generated the elongated and enlarged mitochondria which could not be eliminated via the mitophagy. Three mutant strains displayed the reduced ATP synthesis and vegetative growth compared with the wild type. Three genes were involved in the early stage of conidiation and unnecessary for the late stage. However, all three genes significantly contribute to blastospore development under submerged condition, and the loss ofBbMdv1had the greatest effects compared with the losses ofBbFis1orBbDnm1. Finally, disruption of three genes significantly attenuated fungal virulence, but their mutations had different influences. Conclusions In addition to their consistent roles in mitochondrial division and mitophagy, three fission-related genes perform divergent roles in the development and virulence of the entomopathogenic fungusB. bassiana. Significance and Impact of the Study This study shows that mitochondrial fission is associated with lifecycle ofB. bassiana. These findings provide information for the manipulation of fungal physiology and facilitate the application of entomopathogenic fungi.