Single-walled carbon nanotube biosensors using aptamers as molecular recognition elements
Single-walled carbon nanotube biosensors using aptamers as molecular recognition elements
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DOI：
10.1021/ja053094r
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发表时间：
2005-08-31
影响因子：
15
通讯作者：
Lee, JO
中科院分区：
文献类型：
--
作者：
So, HM;Won, K;Lee, JO
我们报告的实时检测蛋白质使用单壁纳米管-场效应晶体管为基础的生物传感器，包括DNA适体作为分子识别元件。使用CDI-Tween连接分子将对丝氨酸蛋白凝血酶高度特异性的抗凝血酶适体固定在SWNT-FET的侧壁上。凝血酶适体与SWNT-FET的结合导致阈值栅极电压的100 °偏移，这可能是由于DNA适体的带负电荷的主链。虽然凝血酶溶液的加入导致凝血酶适体固定的SWNT-FET的电导突然降低，但弹性蛋白酶没有观察到明显的变化。
We report the real-time detection of protein using SWNT-FET-based biosensors comprising DNA aptamers as molecular recognition elements. Anti-thrombin aptamers that are highly specific to serine protein thrombin were immobilized on the sidewall of a SWNT-FET using CDI-Tween linking molecules. The binding of thrombin aptamers to SWNT-FETs causes a rightward shift of the threshold gate voltages, presumably due to the negatively charged backbone of the DNA aptamers. While the addition of thrombin solution causes an abrupt decrease in the conductance of the thrombin aptamer immobilized SWNT-FET, no noticeable change was observed with elastase.