In vitro evaluation of alpha-tocopherol loaded carboxymethylcellulose chitosan copolymers as wound dressing materials
In vitro evaluation of alpha-tocopherol loaded carboxymethylcellulose chitosan copolymers as wound dressing materials
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DOI：
10.1080/10667857.2019.1573944
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发表时间：
2019-06-07
影响因子：
3.1
通讯作者：
Mansuroglu, Banu
中科院分区：
文献类型：
--
作者：
Bilgic, Melike Bayindir;Lacin, Nelisa Turkoglu;Mansuroglu, Banu
水凝胶是一种生物聚合物，当暴露在水介质中时，由于其不溶性的三维网络结构，它表现出涉及大量水的膨胀行为，并允许其用于伤口敷料。以N，N′-亚甲基双丙烯酰胺（MBA）为交联剂，过硫酸铵（APS）为引发催化剂，通过化学反应合成了壳聚糖和羧甲基纤维素（CMC）聚合物的水凝胶。通过傅里叶变换红外光谱（FTIR）和BET （Brunauer， Emmet和Teller）分析确定了合成的水凝胶的特征。采用液相色谱/Q-TOF技术，采用ESI-MS测定膜上α -生育酚的释放谱。用3-[4,5-二甲基-2-噻唑]-二苯基溴化四氮唑（MTT）法观察细胞粘附和增殖情况。结果显示，水凝胶装载α -生育酚是有效的刺激愈合过程。
The hydrogel is a biopolymer exhibiting swelling behaviour involving a significant amount of water due to its insoluble, three-dimensional network structure when exposed to the aqueous medium, and allows its use in wound dressings. In the present study, a hydrogel was synthesized from chitosan and carboxymethylcellulose (CMC) polymers as a result of a chemical reaction at which N,N'-methylenebisacrylamide (MBA) was used as a cross-linker and ammonium persulfate (APS) as initiator-catalyst. Characteristic features of the synthesized hydrogel were determined by FTIR (Fourier Transform Infrared Spectroscopy), BET (Brunauer, Emmet and Teller) analyses. Alpha-Tocopherol release profile from the membrane was determined via ESI-MS using LC/Q-TOF. Cell adhesion and proliferation was observed by 3-[4,5-dimethyl-2-thiazolyl]-diphenyl tetrazolium bromide (MTT) method. The results revealed that hydrogels loaded alpha-Tocopherol is efficient for stimulating the healing process.