CHINESE AND AFRICAN EUPHORBIACEAE PLANT-EXTRACTS - MARKEDLY ENHANCING EFFECT ON EPSTEIN-BARR VIRUS-INDUCED TRANSFORMATION
CHINESE AND AFRICAN EUPHORBIACEAE PLANT-EXTRACTS - MARKEDLY ENHANCING EFFECT ON EPSTEIN-BARR VIRUS-INDUCED TRANSFORMATION
复制标题
DOI：
10.1016/0304-3835(83)90155-6
复制
发表时间：
1983-01-01
期刊：
影响因子：
9.7
通讯作者：
ITO, Y
中科院分区：
文献类型：
--
作者：
MIZUNO, F;KOIZUMI, S;ITO, Y
中国和非洲大蓬科植物提取物对eb病毒（EBV）诱导的人淋巴细胞转化有显著增强作用。当5倍。105个脐带血淋巴细胞在EBV暴露后立即接种到半固体琼脂培养基中，在最佳剂量的植物提取物存在下，菌落的数量增加了3-10倍。当一个较小的数字是5。将104个细胞接种，在有植物提取物的情况下观察到转化的菌落，而在没有植物提取物的情况下则没有。从琼脂培养基中采集的菌落均为ebv核抗原（EBNA）阳性，呈现典型的囊胚形态。在未感染ebv的培养物中没有检测到菌落，表明这些植物提取物促进这种淋巴细胞转化需要病毒。在中国南方和热带非洲，大戟科植物被认为是当地的多形性药物，本文讨论了大戟科植物提取物作为辅助因子在鼻咽癌（NPC）和非洲伯基特淋巴瘤（BL）发生中的可能作用。
Chinese and African Euphorbiaceae plant extracts were shown to have a markedly enhancing effect on Epstein-Barr virus (EBV)-induced transformation of human lymphocytes. When 5 .times. 105 cord blood lymphocytes were seeded into the semisolid agar medium immediately after EBV exposure, 3-10 times more colonies developed in the presence of the plant extracts at their optimal doses. When a smaller number of 5 .times. 104 cells were seeded, transformed colonies were also observed in the presence of the plant extracts but not in their absence. All of the colonies picked up from the agar medium were EBV-determined nuclear antigen (EBNA)-positive and showed the typical blastoid morphology. There were no colonies detected in the EBV-uninfected cultures with the extracts, indicating that the virus was required for the promotion by these plant extracts of this lymphocyte transformation. Euphorbiaceae plants are known to be employed as local hedral drugs in southern China and tropical Africa, and the possible role as a co-factor of the plant extracts in the development of nasopharyngeal carcinoma (NPC) and African Burkitt''s lymphoma (BL) is discussed.