Spidroins from the Brazilian spider Nephilengys cruentata (Araneae: nephilidae)
Spidroins from the Brazilian spider Nephilengys cruentata (Araneae: nephilidae)
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DOI：
10.1016/j.cbpb.2007.03.013
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发表时间：
2007-08-01
影响因子：
2.2
通讯作者：
Rech, E. L.
中科院分区：
文献类型：
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作者：
Bittencourt, D.;Souto, B. M.;Rech, E. L.
蜘蛛产生多达六种不同的丝，每一种丝都有特定的生物功能。蜘蛛丝还以其独特的机械性能而闻名。制造具有类似性质的新材料的可能性推动了对这些丝蛋白(蜘蛛)的研究。利用表达序列标签，我们从巴西蜘蛛NePhilengys crentata(蜘蛛目：NePhilidae)的主壶腹形、小壶腹形、鞭状和管状丝腺中鉴定出四种蜘蛛。新的蛋白质序列与以前描述的其他蜘蛛类有很大的相似性，由于存在高度重复的基序(PolyAla，(GA)(N)，(GGX)(N)，(GPGGX)(N))，丙氨酸和甘氨酸含量很高。不同蜘蛛之间的序列也很相似，但管状蜘蛛蛋白除外，它呈现出一种独特的复杂氨基酸序列，含有大量的丝氨酸和少量的甘氨酸。(C)2007 Elsevier Inc.保留所有权利。
Spiders produce up to six different kinds of silk, each one for a specific biological function. Spider silks are also known for their unique mechanical properties. The possibility of producing new materials with similar properties motivated research on these silk proteins (spidroins). Using expression sequence tags, we identified four spidroins produced by major ampullate, minor ampullate, flagelliform and tubuliform silk glands from the Brazilian spider Nephilengys cruentata (Araneae: Nephilidae). The new protein sequences showed substantial similarity to other spidroins previously described, with high content of alanine and glycine due to the presence of the highly repetitive motifs (polyAla, (GA)(n), (GGX)(n), (GPGGX)(n)). Similarities among sequences were also observed between the different spidroins with the exception of tubuliform spidroin, which presents a unique complex amino acid sequence with high amounts of serine and low amounts of glycine. (c) 2007 Elsevier Inc. All rights reserved.