PsSAK1, a Stress-Activated MAP Kinase of Phytophthora sojae, Is Required for Zoospore Viability and Infection of Soybean
PsSAK1, a Stress-Activated MAP Kinase of Phytophthora sojae, Is Required for Zoospore Viability and Infection of Soybean
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PsSAK1 是大豆疫霉 (Phytophthora sojae) 的一种胁迫激活 MAP 激酶，是游动孢子活力和大豆感染所必需的
DOI：
10.1094/mpmi-23-8-1022
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发表时间：
2010-08-01
影响因子：
3.5
通讯作者：
Wang, Yuanchao
中科院分区：
文献类型：
--
作者：
Li, Aining;Wang, Yonglin;Wang, Yuanchao
丝裂原活化蛋白激酶（MAPK）通路是所有真核细胞中普遍存在的、进化上保守的信号转导模块。在这项研究中，PsSAK1，其中编码的压力激活MAPK的大豆疫霉，被确定。PsSAK1在卵菌中高度保守，由于其pleckstrin同源结构域而代表了一组新的MAPK。逆转录聚合酶链反应分析表明，PsSAK1的表达上调游动孢子和孢囊，并在感染早期。此外，它的表达诱导的渗透胁迫和氧化应激介导的NaCl和H2O2，分别。为了阐明该功能，使用大豆疫霉的稳定转化沉默PsSAK 1的表达。PsSAK1的沉默不损害菌丝生长、孢子形成或卵孢子产生，但严重阻碍游动孢子发育，因为沉默的菌株比野生型表现出更快的包囊形成和更低的发芽率。PsSAK1沉默突变体产生更长的芽管，不能殖民受伤或未受伤的大豆叶片。我们的研究结果表明PsSAK 1是大豆疫霉游动孢子发育和致病性的重要调节因子。
Mitogen-activated protein kinase (MAPK) pathways are universal and evolutionarily conserved signal transduction modules in all eukaryotic cells. In this study, PsSAK1, which encodes a stress-activated MAPK of Phytophthora sojae, was identified. PsSAK1 is highly conserved in oomycetes, and it represents a novel group of MAPK due to its pleckstrin homology domain. Reverse-transcription polymerase chain reaction analysis showed that PsSAK1 expression was upregulated in zoospores and cysts and during early infection. In addition, its expression was induced by osmotic and oxidative stress mediated by NaCl and H2O2, respectively. To elucidate the function, the expression of PsSAK1 was silenced using stable transformation of P. sojae. The silencing of PsSAK1 did not impair hyphal growth, sporulation, or oospore production but severely hindered zoospore development, in that the silenced strains showed quicker encystment and a lower germination ratio than the wild type. PsSAK1-silenced mutants produced much longer germ tubes and could not colonize either wounded or un-wounded soybean leaves. Our results indicate that PsSAK1 is an important regulator of zoospore development and pathogenicity in P. sojae.