Misuse of nonlinear Scatchard plots.
Misuse of nonlinear Scatchard plots.
复制标题
滥用非线性斯卡查德图。
DOI：
10.1016/0968-0004(89)90157-6
复制
发表时间：
1989
影响因子：
13.8
通讯作者：
Zierler,K
中科院分区：
文献类型：
--
作者：
Zierler,K
Scatchard图-结合/自由配体与结合配体的关系图-是结合数据的常见图形表示。它们往往是非线性的。尽管有正确用法的例子和几篇文章提醒人们对Scatchard曲线图的错误处理，但对非线性Scatchard曲线图的错误解释仍然很常见；曲线图被错误地分解为两个或更多与可接受的结合模型无关的线性分量。正确的分析通常需要通过计算机确定结合参数的数值，该数值给出了适当模型的最佳非线性拟合度，并给出了具体的例子。
Scatchard plots - plots of bound/free ligand vs bound ligand - are a common graphical presentation of binding data. They are often nonlinear. Despite examples of correct usage and several articles calling attention to incorrect treatment of Scatchard plots, erroneous interpretations of nonlinear Scatchard plots remain frequent; plots are resolved incorrectly into two or more linear components which have no relation to an acceptable binding model. Correct analysis requires determination, usually by computer, of numerical values of the binding parameters that give the best nonlinear fit to an appropriate model, examples of which are specified.