Differentiation of human embryonic stem cells along a hepatocyte lineage and its application in liver regeneration
Differentiation of human embryonic stem cells along a hepatocyte lineage and its application in liver regeneration
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DOI：
10.1007/s11434-008-0026-x
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发表时间：
2008
影响因子：
--
通讯作者：
Haiyun Pei;Yunfang Wang;Xuetao Pei
中科院分区：
文献类型：
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作者：
Haiyun Pei;Yunfang Wang;Xuetao Pei
肝细胞移植和生物人工肝（BAL）作为肝移植的替代方案，为许多遗传性和获得性肝脏疾病提供了有效治疗的可能性。不幸的是，捐赠肝脏的有限可用性及其衍生肝细胞的可变性使得难以获得足够的活的人肝细胞用于肝细胞为基础的治疗。胚胎干细胞（ESCs）可直接从囊胚内细胞团中分离出来，具有永久的自我更新能力和发育多能性，可能是治疗肝功能不全的理想细胞来源。然而，将hESCs分化为大量的肝细胞仍然是一个挑战。这篇综述更新了我们目前对ESCs向肝系细胞分化的理解，它们未来的治疗用途和肝脏再生中的问题。
Hepatocyte transplantation and bioartificial liver (BAL) as alternatives to liver transplantation offer the possibility of effective treatment for many inherited and acquired hepatic disorders. Unfortunately, the limited availability of donated livers and the variability of their derived hepatocytes make it difficult to obtain enough viable human hepatocytes for the hepatocyte-based therapies. Embryonic stem cells (ESCs), which could be isolated directly from the blastocyst inner cell mass, have permanent self-renewal capability and developmental pluripotency and therefore might be an ideal cell source in the treatment of hepatic discords. However, differentiation of hESCs into hepatocytes with significant numbers remains a challenge. This review updates our current understanding of differentiation of ESCs into hepatic lineage cells, their future therapeutic uses and problems in liver regeneration.