Antigenic variation during persistent infection by equine infectious anemia virus, a retrovirus.
Antigenic variation during persistent infection by equine infectious anemia virus, a retrovirus.
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马传染性贫血病毒（一种逆转录病毒）持续感染期间的抗原变异。
DOI：
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发表时间：
1984
影响因子：
4.8
通讯作者：
C. Issel
中科院分区：
文献类型：
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作者：
R. Montelaro;B. Parekh;A. Orrego;C. Issel
马传染性贫血的复发本质是由于在选择性免疫压力下持续感染期间马传染性贫血病毒(EIAV)的相对快速的抗原变异。通过对从两匹实验感染的小马的不同发热期中回收的病毒分离株的血清学和生化分析，对该模型进行了测试。用实验感染的小马的免疫血清进行的中和分析表明，在单个小马的连续发烧期间，不同的抗原株病毒占主导地位。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法对EIAV测试毒株进行分析，发现病毒粒子糖蛋白gp90和gp45的电泳率不同。此外，肽图谱比较表明，不同菌株的gp90组分之间存在结构差异。相反，病毒株各自的内部蛋白在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法中表现出相同的电泳率，并且通常产生相同的胰蛋白酶多肽图谱。观察到的糖蛋白电泳率和多肽图谱的差异是高度重复性的，并且不会随着病毒株在细胞培养中的反复传代而改变。因此，这些结果证明了在EIAV持续感染期间发生了糖蛋白特异性结构变异，并支持这种逆转录病毒的抗原变异的概念。这种改变包膜糖蛋白结构的能力，以前报道过的Visna病毒，可能代表了逆转录病毒在动物宿主免疫反应存在时持续存在的重要机制。
The recurrent nature of equine infectious anemia has been attributed to relatively rapid antigenic variations in equine infectious anemia virus (EIAV) during persistent infection under selective immune pressures. This model was tested by serological and biochemical analysis of virus isolates recovered from separate febrile episodes in two experimentally infected ponies. Neutralization assays employing immune sera from the experimentally infected ponies demonstrated that distinct antigenic strains of virus predominate during sequential febrile episodes in a single pony. Analysis of the test strains of EIAV by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed varying electrophoretic mobilities for the respective virion glycoproteins gp90 and gp45. Furthermore, peptide mapping comparisons demonstrated structural variations between the gp90 components of the various strains. In contrast, the respective internal proteins of the virus strains displayed identical electrophoretic mobilities in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and, in general, produced identical tryptic peptide maps. The observed differences in glycoprotein electrophoretic mobility and peptide maps were highly reproducible and did not vary with repeated passage of the virus strains in cell culture. Thus, these results demonstrate the occurrence of glycoprotein-specific structural variations during persistent infection by EIAV and support the concept of antigenic variation in this retrovirus. This capacity to alter envelope glycoprotein structure, previously reported for visna virus, may represent an important mechanism of retrovirus persistence in the presence of immune responses by the animal host.