Isolation and characterisation of a diverse family of Arabidopsis two and three-fingered C2H2 zinc finger protein genes and cDNAs
Isolation and characterisation of a diverse family of Arabidopsis two and three-fingered C2H2 zinc finger protein genes and cDNAs
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DOI：
10.1023/a:1005746803089
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发表时间：
1997-03-01
影响因子：
5.1
通讯作者：
Michael, AJ
中科院分区：
文献类型：
--
作者：
Meissner, R;Michael, AJ
C2 H2锌指蛋白（ZFP）基因家族是动物系统中最大的一类调控蛋白，其成员在动物的生长发育过程中具有广泛而重要的作用。ZFP转录因子家族可能在植物中也很重要。我们已经使用了PCR的方法，采用高度简并的寡核苷酸引物分离几个拟南芥基因组和cDNA克隆编码潜在的ZFP。此外，我们还利用这些克隆的序列信息鉴定了两个ESTs作为该家族的成员。已鉴定出五种双指和一种三指ZFP。在锌指区之外，存在相当大的序列多样性，包括指间区的序列和长度;每个基因的表达模式是不同的。这是首次报道的三指植物C2 H2 ZFP基因的分离，我们讨论了其可能的进化起源于两指ZFP基因。
In animal systems the C2H2 zinc finger protein (ZFP) gene family is the largest group of regulatory proteins and its members have a wide and important role in growth and development. It is likely that this family of ZFP transcription factors will also be important in plants. We have used a PCR approach employing highly degenerate oligonucleotide primers to isolate several Arabidopsis genomic and cDNA clones encoding potential ZFPs. In addition we have used the sequence information from these clones to identify two ESTs as members of this family. Five two-fingered and one three-fingered ZFPs have been identified. Outside of the zinc finger regions, there is considerable sequence diversity, including the sequence and length of the interfinger region; the expression pattern of each gene is different. This is the first report of the isolation of a three-fingered plant C2H2 ZFP gene and we discuss its possible evolutionary origin from two-fingered ZFP genes.