Effect of voluntary exercise on H2O2 release by subsarcolemmal and intermyofibrillar mitochondria
Effect of voluntary exercise on H2O2 release by subsarcolemmal and intermyofibrillar mitochondria
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DOI：
10.1016/s0891-5849(03)00177-1
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发表时间：
2003-07-01
影响因子：
7.4
通讯作者：
Favier, R
中科院分区：
文献类型：
--
作者：
Servais, S;Couturier, K;Favier, R
先前的数据表明，为了应对高强度训练中遇到的氧化应激，骨骼肌依赖于线粒体生物合成的增加、H2O2 产生的减少以及抗氧化酶的增强。在本研究中，我们评估了自愿跑步对从氧化性肌肉中分离的肌原纤维间线粒体（IFM）和肌膜下线粒体（SSM）线粒体O-2消耗和H2O2产生的影响，并结合抗氧化能力的测定。当线粒体与琥珀酸作为底物孵育时，SSM 中的最大（状态 3）和静息（状态 4）O-2 消耗均显着低于 IFM 群体。 SSM 中每单位 O-2 消耗的线粒体 H2O2 释放量是 EFM 中的 2 倍。鱼藤酮对 H2O2 形成的抑制表明电子传递链的复合物 I 可能是主要的生理性 H2O2 生成系统。在 Lou/C 大鼠（Wistar 大鼠的近交系）中，IFM 和 SSM 的 O-2 消耗和 H2O2 释放均不受长期、自愿的轮训练的影响。相比之下，尽管长期自愿运动的氧化能力没有变化，但谷胱甘肽过氧化物酶和过氧化氢酶活性显着增加。此外，长期运动会增强骨骼肌内热休克蛋白 72 的积累。结论是可以显着改善肌肉的抗氧化状态。通过长时间的轮运动而无需增加线粒体氧化能力。 (C) 2003 爱思唯尔公司。
Previous data have demonstrated that, to handle the oxidative stress encountered with training at high intensity, skeletal muscle relies on an increase in mitochondrial biogenesis, a reduced H2O2 production, and an enhancement of antioxidant enzymes. In the present study, we evaluated the influence of voluntary running on mitochondrial O-2 consumption and H2O2 production by intermyofibrillar mitochondria (IFM) and subsarcolemmal mitochondria (SSM) isolated from oxidative muscles in conjunction with the determination of antioxidant capacities. When mitochondria are incubated with succinate as substrate, both maximal (state 3) and resting (state 4) O-2 consumption were significantly lower in SSM than in IFM populations. Mitochondrial H2O2 release per unit of O-2 consumed was 2-fold higher in SSM than in EFM. Inhibition of H2O2 formation by rotenone suggests that complex I of the electron transport chain is likely the major physiological H2O2-generating system. In Lou/C rats (an inbred strain of rats of Wistar origin), neither O-2 consumption nor H2O2 release by IFM and SSM were affected by long-term, voluntary wheel training. In contrast, glutathione peroxidase and catalase activity were significantly increased despite no change in oxidative capacities with long-term, voluntary exercise. Furthermore, chronic exercise enhanced heat shock protein 72 accumulation within skeletal muscle. It is concluded that the antioxidant status of muscle can be significantly improved. by prolonged wheel exercise without necessitating an increase in mitochondrial oxidative capacities. (C) 2003 Elsevier Inc.