Increased concentrations of myeloperoxidase in serum and serum extracellular vesicles are associated with type 2 diabetes mellitus
Increased concentrations of myeloperoxidase in serum and serum extracellular vesicles are associated with type 2 diabetes mellitus
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血清和血清细胞外囊泡中髓过氧化物酶浓度升高与 2 型糖尿病相关
DOI：
10.1016/j.cca.2021.08.010
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发表时间：
2021-08-22
影响因子：
5
通讯作者：
Zhang, Huina
中科院分区：
文献类型：
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作者：
Peng, Lu;Li, Xinwei;Zhang, Huina
背景：炎症反应在2型糖尿病(T2 DM)的发生发展中起着重要作用。髓过氧化物酶(MPO)是一种白细胞衍生的主药，在许多并发症中发挥其促炎作用。我们探讨了血清细胞外小泡(EV)来源的MPO以及血清MPO与T2 DM之间的关系。方法：我们对151名个体进行了横断面研究，包括93名T2 DM患者和58名非T2 DM对照组。Luminex比色法测定血清EV源性MPO和血清MPO浓度。结果：T2 DM组血清EV源性MPO浓度和血清MPO浓度均显著高于非T2 DM组。多因素Logistic回归分析显示，即使在调整混杂因素后，血清EV源性MPO和血清MPO仍与T2 DM独立相关(OR=1.836/1 ng EV源性MPO，95%CI=1.395~2.417，P<0.001；OR=4.135/10 ng血清MPO，95%CI=2.285~7.483，P<0.001)。此外，在筛查T2 DM时，血清MPO的判别准确率略高于EV来源的MPO(AUC=0.858；AUC=0.779)。结论：血清或血清EVS中炎性标志物MPO浓度升高与T2 DM独立相关。
Background: Inflammatory response plays a critical role in the initiation and progression of type 2 diabetes mellitus (T2DM). Myeloperoxidase (MPO), a leukocyte-derived protagonist, exerts its proinflammatory properties in many complications. We explored the associations between serum extracellular vesicle (EV)-derived MPO as well as serum MPO and T2DM. Methods: We performed a cross-sectional study in 151 individuals, including 93 patients with T2DM and 58 nonT2DM controls. The concentrations of serum EV-derived MPO and serum MPO were measured by Luminex Assay. Results: Our data showed that serum EV-derived MPO concentrations and serum MPO concentrations were significantly higher in T2DM patients compared with non-T2DM subjects. In addition, multivariate logistic regression analysis revealed that serum EV-derived MPO as well as serum MPO was independently associated with the presence of T2DM even after adjusting for confounding factors (OR = 1.836 /1 ng EV-derived MPO, 95% CI = 1.395-2.417, P < 0.001; OR = 4.135 /10 ng serum MPO, 95% CI = 2.285-7.483, P < 0.001). Furthermore, serum MPO showed marginally higher discriminatory accuracy than serum EV-derived MPO in screening T2DM (AUC = 0.858; AUC = 0.779). Conclusion: Increased concentrations of the inflammatory marker MPO either in serum or in serum EVs were independently associated with T2DM.