COLLOIDAL GOLD PARTICLES AS AN INCOMPRESSIBLE ATOMIC-FORCE MICROSCOPE IMAGING STANDARD FOR ASSESSING THE COMPRESSIBILITY OF BIOMOLECULES
COLLOIDAL GOLD PARTICLES AS AN INCOMPRESSIBLE ATOMIC-FORCE MICROSCOPE IMAGING STANDARD FOR ASSESSING THE COMPRESSIBILITY OF BIOMOLECULES
复制标题
DOI：
10.1016/s0006-3495(93)81171-8
复制
发表时间：
1993-09-01
影响因子：
3.4
通讯作者：
HENDERSON, E
中科院分区：
文献类型：
--
作者：
VESENKA, J;MANNE, S;HENDERSON, E
胶体金颗粒作为三维原子力显微镜成像标准品具有多种用途，因为它们是不可压缩的、单分散的和球形的。颗粒的球形性质可用于表征扫描尖端几何形状。作为均匀的球体，胶体金颗粒可用于在纳米水平上校准原子力显微镜的垂直尺寸。金的单分散性和不可压缩性可用于表征共吸附生物分子的垂直尺寸。同时测量金与烟草花叶病毒表明，在相同的垂直力，烟草花叶病毒是不受损害的钝尖，但被压缩或粉碎下更尖锐的扫描探针，这表明标本降解部分是一个压力依赖性的效果。
Colloidal gold particles have multiple uses as three-dimensional atomic force microscopy imaging standards because they are incompressible, monodisperse, and spherical. The spherical nature of the particles can be exploited to characterize scanning tip geometry. As uniform spheres, colloidal gold particles may be used to calibrate the vertical dimensions of atomic force microscopy at the nanometer level. The monodisperse and incompressible nature of the gold can be used to characterize the vertical dimensions of coadsorbed biomolecules. Simultaneous measurements of gold with tobacco mosaic virus show that, at the same applied vertical force, the tobacco mosaic virus is undamaged by blunt tips but is compressed or disintegrated under sharper scanning styli, suggesting that specimen degradation is partly a pressure-dependent effect.