Measuring the Surface-Surface Interactions Induced by Serum Proteins in a Physiological Environment.
Measuring the Surface-Surface Interactions Induced by Serum Proteins in a Physiological Environment.
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DOI：
10.1021/acs.langmuir.6b03420
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发表时间：
2016-11
期刊：
影响因子：
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通讯作者：
Zhaohui Wang;Chuanxin He;Xiangjun Gong;Jianqi Wang;To Ngai
中科院分区：
文献类型：
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作者：
Zhaohui Wang;Chuanxin He;Xiangjun Gong;Jianqi Wang;To Ngai
在这项工作中，我们应用全内反射显微镜(TIRM)直接测量了三种不同的宏观表面之间的相互作用：即在磷酸盐缓冲溶液(PBS)和胎牛血清(FBS)中，在磷酸盐缓冲溶液(PBS)和胎牛血清(FBS)中，PS颗粒和裸SiO_2(裸-PS/裸-SiO_2)表面，PS颗粒和SiO_2表面都包覆了牛血清白蛋白(BSA-PS/BSA-SiO_2)，PS颗粒和SiO_2表面都被聚乙二醇改性(PEG-PS/PEG-SiO_2)聚合物修饰。结果表明，在PBS中，裸PS、BSA-PS和PEGPS颗粒都不可逆地沉积在裸的二氧化硅表面或涂有BSA或PEG膜的表面。然而，在FBS中，颗粒与表面之间的相互作用势既表现为自由扩散的颗粒轮廓，也表现为粘着的颗粒轮廓。动态光散射(DLS)和椭圆仪测量表明，PS颗粒和二氧化硅表面吸附了一层血清蛋白。TIRM测量表明，这种吸附的血清蛋白可以在一定条件下提供额外的稳定，但也促进了两个表面之间的桥梁效应，从而介导了表面-表面的相互作用。因此，在FBS中测得的滞留粒子的电势分布比在PbS中测得的要宽得多。这些定量测量提供了一些见解，即吸附在表面上的血清蛋白可以调节表面-表面的相互作用，从而导致胶体颗粒在血清环境中独特的运动行为和稳定性。
In this work, we applied total internal reflection microscopy (TIRM) to directly measure the interactions between three different kinds of macroscopic surfaces: namely bare polystyrene (PS) particle and bare silica surface (bare-PS/bare-silica), PS particle and silica surfaces both coated with bovine serum albumin (BSA) (BSA-PS/BSA-silica), and PS particle and silica surfaces both modified with polyethylene glycol (PEG) (PEG-PS/PEG-silica) polymers, in phosphate buffer solution (PBS) and fetal bovine serum (FBS). Our results showed that in PBS, all the bare-PS, BSA-PS, and PEG-PS particles were irreversibly deposited onto the bare silica surface or surfaces coated either with BSA or PEG. However, in FBS, the interaction potentials between the particle and surface exhibited both free-diffusing particle and stuck particle profiles. Dynamic light scattering (DLS) and elliposmeter measurements indicated that there was a layer of serum proteins adsorbed on the PS particle and silica surface. TIRM measurement revealed that such adsorbed serum proteins can mediate the surface-surface interactions by providing additional stabilization under certain conditions, but also promoting bridging effect between the two surfaces. The measured potential profile of the stuck particle in FBS thus was much wider than in PBS. These quantitative measurements provide insights that serum proteins adsorbed onto surfaces can regulate surface-surface interactions, thus leading to unique moving behavior and stability of colloidal particles in the serum environment.