Autotetraploids and genetic mapping using common AFLP markers:: the R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4
Autotetraploids and genetic mapping using common AFLP markers:: the R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4
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DOI：
10.1007/s001220050847
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发表时间：
1998-06-01
影响因子：
5.4
通讯作者：
Jacobsen, E
中科院分区：
文献类型：
--
作者：
Li, X;van Eck, HJ;Jacobsen, E
由于四体遗传的复杂性，马铃薯（Solanum tuberosum L.）的图谱研究通常在二倍体水平上进行。在本研究中，我们测试了使用四倍体后代进行批量分离分析 (BSA) 的可行性，以鉴定与 R2 等位基因连锁的 AFLP 标记，该标记赋予对致病疫霉的种族特异性抗性。在 205 个 AFLP 引物组合的指纹中检测到的 11 个批量特异性 AFLP 标记可以映射到包含 R2 基因座的连锁群中。由单剂量限制性片段 (SDRF) 的频率决定的四倍体水平的 BSA 效率远高于基于亲本克隆之间总体遗传差异的预期。与 R2 等位基因连锁的 AFLP 的偶然检测是基于栽培马铃薯与携带抗性 R2 等位基因的 S. demissum 基因渗入的染色体片段之间的特定遗传差异来解释的。与 R2 连锁的标记共有的 AFLP 标记通过其在参考作图群体的亲代完成的 AFLP 指纹中的电泳迁移率在视觉上被识别。使用这些常见的 AFLP 标记，我们将包含 R2 等位基因的连锁群锚定到马铃薯 4 号染色体上。
Due to the complexity of tetrasomic inheritance, mapping studies in potato (Solanum tuberosum L.) are generally conducted at the diploid level. In the present study we tested the feasibility of Bulked Segregant Analysis (BSA) using a tetraploid offspring for the identification of AFLP markers linked to the R2 allele, which confers race-specific resistance to Phytophthora infestans. Eleven bulk-specific AFLP markers, detected in fingerprints of 205 AFLP primer combinations, could be mapped in a linkage group encompassing the R2 locus. The efficiency of BSA at the tetraploid level, determined by the frequency of single-dose restriction fragments (SDRF), was much higher than expected on the basis of overall genetic dissimilarity between the parental clones. The fortuitous detection of AFLPs with linkage to the R2 allele is explained on the basis of specific genetic dissimilarity between cultivated potato and the chromosomal segment introgressed from S. demissum carrying the resistant R2 allele. AFLP markers common to those with linkage to R2 were visually recognized by their electrophoretic mobility in the AFLP fingerprint in a parental done of a reference mapping population. Using these common AFLP markers we anchored the linkage group comprising the R2 allele to potato chromosome 4.