Phenotypic and phylogenetic segregation of Alternaria infectoria from small-spored Alternaria species isolated from wheat in Germany and Russia
Phenotypic and phylogenetic segregation of Alternaria infectoria from small-spored Alternaria species isolated from wheat in Germany and Russia
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DOI：
10.1111/jam.12951
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发表时间：
2015-12-01
影响因子：
4
通讯作者：
Mueller, M. E. H.
中科院分区：
文献类型：
--
作者：
Kahl, S. M.;Ulrich, A.;Mueller, M. E. H.
目的：通过多相方法鉴定从德国和俄罗斯小麦籽粒中分离出的植物病原小孢子链格孢属菌株之间的分类学差异。方法和结果：根据翻译延伸因子 1-α (TEF1-alpha) 的序列变异，对 95 个链格孢属 (A.) 菌株的菌落颜色、三维孢子形成模式、霉菌毒素产生和系统发育关系进行了表征。通过 TEF1-α 对毒素谱和系统发育特征的检查产生了两个不同的簇，在每种情况下，第一个簇中包含 Alternaria infectoria 分离株（分别为 92% 和 96%），而另一个组合簇中包含 Alternaria alternata、Alternaria arborescens 和 Alternaria tenuissima 分离株（分别为 77% 和 79%）。此前未曾报道过食环孢菌物种组中链格孢醇、链格孢毒素和链格孢烯的产生。来自德国和俄罗斯的分离株在物种组成和霉菌毒素生产能力方面略有不同。结论：我们确定，没食子菌物种组可以通过颜色、低霉菌毒素产量和 TEF1-α 基因的序列变异与 A. alternata、A. arborescens 和 A. tenuissima 物种组区分开来。 研究的意义和影响：这些结果可以在检测谷物上不同的链格孢属真菌时进行可靠的毒性风险评估。
Aims: To identify the taxonomic differences between phytopathogenic smallspored Alternaria strains isolated from wheat kernels in Germany and Russia by a polyphasic approach.Methods and Results: Ninety-five Alternaria (A.) strains were characterized by their colony colour, their three-dimensional sporulation patterns, mycotoxin production and phylogenetic relationships based on sequence variation in translation elongation factor 1-alpha (TEF1-alpha). The examination of toxin profiles and the phylogenetic features via TEF1-alpha resulted in two distinct clusters, in each case containing Alternaria infectoria isolates (92 and 96% respectively) in the first and the Alternaria alternata, Alternaria arborescens and Alternaria tenuissima isolates (77 and 79% respectively) in the other combined cluster. The production of Alternariol, Altertoxin and Altenuene has not been reported previously in the A. infectoria species group. The isolates from Germany and Russia differ slightly in species composition and mycotoxin production capacity.Conclusions: We identified that the A. infectoria species group can be differentiated from the A. alternata, A. arborescens and A. tenuissima species group by colour, low mycotoxin production and by the sequence variation in TEF1-alpha gene.Significance and Impact of the Study: These results allow a reliable toxic risk assessment when detecting different Alternaria fungi on cereals.