Transcriptional response to a prime/boost vaccination of chickens with three vaccine variants based on HA DNA and Pichia-produced HA protein
Transcriptional response to a prime/boost vaccination of chickens with three vaccine variants based on HA DNA and Pichia-produced HA protein
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DOI：
10.1016/j.dci.2018.07.001
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发表时间：
2018-11-01
影响因子：
2.9
通讯作者：
Sirko, Agnieszka
中科院分区：
文献类型：
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作者：
Kalenik, Barbara Malgorzata;Gora-Sochacka, Anna;Sirko, Agnieszka
高致病性禽流感造成严重的经济损失，对公众健康构成潜在威胁。更好地了解鸡对新型禽流感疫苗的反应机制可能有助于在不同国家的家禽疫苗接种计划中成功实施。这项工作提出了一个全面的分析，在鸡脾脏中引起的基因表达反应的DNA/重组蛋白组合的启动/加强疫苗相比，DNA/DNA和蛋白质/蛋白质方案。与对照组相比，所有免疫组鸡的脾脏转录组均显示出变化，在蛋白/蛋白、DNA/DNA和DNA/蛋白组中分别鉴定出423、375和212个差异表达基因。具有最显著表达变化的基因属于免疫相关类别。根据组的不同，所有组共有15-34%的上调免疫相关基因和15-42%的下调免疫相关基因。有趣的是，上调最多的基因编码β-防御素，具有抗微生物活性和免疫调节功能的短肽。用RT-qPCR方法验证了微阵列结果，证实了所选免疫相关基因的差异调控。免疫相关的差异表达基因和代谢途径，在这项工作中确定的基因表达的变化，在接种疫苗和未接种疫苗的鸡流感感染后的可用文献数据进行比较。
Highly pathogenic avian influenza causes severe economic losses and is a potential threat to public health. Better knowledge of the mechanisms of chicken response to the novel types of vaccines against avian influenza might be helpful in their successful implementation into poultry vaccination programs in different countries. This work presents a comprehensive analysis of gene expression response elicited in chicken spleens by a combined DNA/recombinant protein prime/boost vaccination compared to DNA/DNA and protein/protein regimens. All groups of vaccinated chickens displayed changes in spleen transcriptomes in comparison to the control group with 423, 375 and 212 identified differentially expressed genes in protein/protein, DNA/DNA and DNA/protein group, respectively. Genes with most significantly changed expression belong to immune-related categories. Depending on a group, a fraction of 15-34% of up-regulated and a fraction of 15-42% of down-regulated immune-related genes are shared by all groups. Interestingly, the most upregulated genes encode beta-defensins, short peptides with antimicrobial activity and immunomodulatory functions. Microarray results were validated with RT-qPCR method, which confirmed differential regulation of the selected immune-related genes. Immune-related differentially expressed genes and metabolic pathways identified in this work are compared to the available literature data on gene expression changes in vaccinated and non-vaccinated chickens after influenza infection.