The 5'-terminal region of a tombusvirus genome determines the origin of multivesicular bodies
The 5'-terminal region of a tombusvirus genome determines the origin of multivesicular bodies
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DOI：
10.1099/0022-1317-77-8-1967
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发表时间：
1996-08-01
影响因子：
3.8
通讯作者：
Russo, M
中科院分区：
文献类型：
--
作者：
Burgyan, J;Rubino, L;Russo, M
多泡体（MVB）是一种膜质细胞质内含物，无论病毒种类、宿主或被检查的组织如何，都与瘤状病毒感染有关。MVB是病毒诱导的结构，因为它们不存在于健康植物的组织中，但总是存在于被感染的植物和原生质体中。MVB来源于被兰花环斑病毒（Gym RSV）感染的细胞的过氧化物酶体和被香石竹意大利环斑病毒（CIRV）感染的细胞的线粒体，利用全长感染性克隆中常见的限制性内切位点，构建了这两种病毒的杂交克隆，并制备了CIRV的突变体，该突变体中第一开放阅读框编码的蛋白缩短了22个氨基酸。所有的突变转录本都是活的，并感染了本烟植物，收集了感染的叶片组织样本，进行薄切片处理，并在电镜下观察，发现MVB的起源受病毒基因组5'区控制，ORF 1中约600个核苷酸的短序列包含了由过氧化物酶体或线粒体形成MVB的决定因素。
Multivesicular bodies (MVB) are membranous cytoplasmic inclusions that are invariably associated with tombusvirus infections regardless of the virus species, the host, or the tissue examined, MVB are virus-induced structures since they are absent from tissues of healthy plants and are always present both in infected plants and protoplasts, MVB derive from peroxisomes in cells infected by a number of tombusviruses including cymbidium ringspot virus (Gym RSV) and from mitochondria in cells infected by another tombusvirus, carnation Italian ringspot virus (CIRV), By using common restriction sites in full-length infectious clones, hybrid clones of these two viruses were constructed, In addition, a mutant of CIRV was prepared in which the protein encoded by the first open reading frame was shortened by 22 amino acids, All mutant transcripts were viable and infected Nicotiana benthamiana plants, Infected leaf tissue samples were collected, processed for thin sectioning, and observed in the electron microscope, The origin of MVB was shown to be under the control of the 5' region of the viral genome, A sequence as short as about 600 nucleotides in ORF 1 contained the determinants for formation of MVB from peroxisomes or mitochondria.