Transcription Repressor HANABA TARANU Controls Flower Development by Integrating the Actions of Multiple Hormones, Floral Organ Specification Genes, and GATA3 Family Genes in Arabidopsis
Transcription Repressor HANABA TARANU Controls Flower Development by Integrating the Actions of Multiple Hormones, Floral Organ Specification Genes, and GATA3 Family Genes in Arabidopsis
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转录抑制因子 HANABA TARANU 通过整合拟南芥中多种激素、花器官规格基因和 GATA3 家族基因的作用来控制花发育
DOI：
10.1105/tpc.112.107854
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发表时间：
2013-01-01
期刊：
影响因子：
11.6
通讯作者：
Meyerowitz, Elliot M.
中科院分区：
文献类型：
--
作者：
Zhang, Xiaolan;Zhou, Yun;Meyerowitz, Elliot M.
植物花序分生组织和花分生组织具有特定的边界域，导致适当的花器官分离和规格。HANABA TARANU（HAN）编码一个边界表达的GATA 3型转录因子，该因子调控拟南芥茎分生组织的形成和花的发育，但其内在机制尚不清楚。通过对HAN瞬时过表达后的时间进程微阵列分析，我们发现HAN抑制了数百个基因，特别是参与激素反应和花器官特化的基因。HAN的瞬时过表达还抑制HAN和其他三种GATA 3家族基因HANL 2（HAN-LIKE 2）、GNC（加塔，硝酸盐诱导，碳代谢相关）和GNL（GNC-LIKE）的表达，形成负调节反馈环。遗传分析表明，HAN和GATA 3家族的3个基因协同调控花的发育，并且它们的表达模式有部分重叠。HAN可以与这些基因编码的三种蛋白质同源二聚体化和异源二聚体化，并且HAN在体内直接与其自身的启动子和GNC启动子结合。这些发现，以及HAN组成性过表达产生比功能丧失突变更强的表型的事实，沿着以下假设：HAN作为关键抑制因子发挥作用，通过涉及GATA 3家族基因的调节网络来调节花发育，沿着参与激素作用和花器官特化的基因。
Plant inflorescence meristems and floral meristems possess specific boundary domains that result in proper floral organ separation and specification. HANABA TARANU (HAN) encodes a boundary-expressed GATA3-type transcription factor that regulates shoot meristem organization and flower development in Arabidopsis thaliana, but the underlying mechanism remains unclear. Through time-course microarray analyses following transient overexpression of HAN, we found that HAN represses hundreds of genes, especially genes involved in hormone responses and floral organ specification. Transient overexpression of HAN also represses the expression of HAN and three other GATA3 family genes, HANL2 (HAN-LIKE 2), GNC (GATA, NITRATE-INDUCIBLE, CARBON-METABOLISM-INVOLVED), and GNL (GNC-LIKE), forming a negative regulatory feedback loop. Genetic analysis indicates that HAN and the three GATA3 family genes coordinately regulate floral development, and their expression patterns are partially overlapping. HAN can homodimerize and heterodimerize with the three proteins encoded by these genes, and HAN directly binds to its own promoter and the GNC promoter in vivo. These findings, along with the fact that constitutive overexpression of HAN produces an even stronger phenotype than the loss-of-function mutation, support the hypothesis that HAN functions as a key repressor that regulates floral development via regulatory networks involving genes in the GATA3 family, along with genes involved in hormone action and floral organ specification.