MULLERIAN INHIBITING SUBSTANCE MESSENGER-RIBONUCLEIC-ACID EXPRESSION IN GRANULOSA AND SERTOLI CELLS COINCIDES WITH THEIR MITOTIC-ACTIVITY
MULLERIAN INHIBITING SUBSTANCE MESSENGER-RIBONUCLEIC-ACID EXPRESSION IN GRANULOSA AND SERTOLI CELLS COINCIDES WITH THEIR MITOTIC-ACTIVITY
复制标题
DOI：
10.1210/en.131.2.854
复制
发表时间：
1992-08-01
期刊：
影响因子：
4.8
通讯作者：
DONAHOE, PK
中科院分区：
文献类型：
--
作者：
HIROBE, S;HE, WW;DONAHOE, PK
在男性，苗勒抑制物质（MIS）的mRNA首先检测到的内侧方面的泌尿生殖嵴早在早上的第13天的妊娠睾丸分化是明显的，并定位到更明显的支持细胞后，胚胎第13天。MIS成绩单保持在最高水平之间的14.5和17.5天妊娠，而苗勒管内卷，并保持高水平，直到出生。出生后MIS基因表达逐渐下降，随着生殖细胞减数分裂的增加，在生精小管的支持细胞中几乎检测不到。在雌性大鼠中，MIS mRNA首先在出生后3天围绕较大初级卵泡的单层立方颗粒细胞中检测到，与卵泡生长的开始一致。随着卵泡生长的进行，MIS mRNA在腔前卵泡和小腔卵泡中表达较高，尤其是在最靠近卵母细胞的颗粒细胞中。MIS mRNA的表达逐渐下降，在较大的窦卵泡，仍然突出，只有在卵丘细胞和最接近管腔的颗粒细胞的分裂人口。MIS基因的表达是不存在的卵泡闭锁的功能，并在较大的窦卵泡。MIS mRNA在活跃分裂的支持细胞和颗粒细胞中的表达与生殖细胞分裂的阶段相关。这些发现提示MIS在控制生殖细胞成熟中的作用。
In males, Mullerian inhibiting substance (MIS) mRNA was first detected on the medial aspect of the urogenital ridge early on the morning of day 13 of gestation before testicular differentiation was evident, and localized to the more obvious Sertoli cells later on embryonic day 13. MIS transcripts remained at maximal levels between 14.5 and 17.5 days gestation, while the Mullerian duct involutes, and remained high until birth. MIS gene expression decreased progressively after birth and, as germ cell meiosis increased, became barely detectable in the Sertoli cells of the seminiferous tubules. In female rats, MIS mRNA was first detected in the single layer of cuboidal granulosa cells surrounding larger primary follicles 3 days after birth, coincident with the initiation of follicular growth. As follicular growth progressed, MIS mRNA expression was high in preantral and small antral follicles, especially in those granulosa cells closest to the oocyte. MIS mRNA expression decreased gradually in larger antral follicles, remaining prominent only in the cumulus cells and the dividing population of granulosa cells closest to the lumen. MIS gene expression was absent in follicles with features of atresia and in the larger antral follicles. The expression of MIS mRNA in actively dividing Sertoli and granulosa cells correlates with the stages of germ cell division. These findings are suggestive of a role for MIS in the control of germ cell maturation.