Gene expression in insecticide resistant and susceptible Anopheles gambiae strains constitutively or after insecticide exposure
Gene expression in insecticide resistant and susceptible Anopheles gambiae strains constitutively or after insecticide exposure
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DOI：
10.1111/j.1365-2583.2005.00582.x
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发表时间：
2005-10-01
影响因子：
2.6
通讯作者：
Ranson, H
中科院分区：
文献类型：
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作者：
Vontas, J;Blass, C;Ranson, H
一个微阵列包含约20 000个表达序列标签（EST; 11 760个独特的EST簇）的疟疾载体，冈比亚按蚊，被用来监测全球基因表达的差异在两个杀虫剂抗性和一个敏感株。统计分析发现，77个ESTs之间的差异转录三株。这些包括细胞色素P450 CYP 314 A1，在DDT抗性ZAN/U菌株中过度转录，以及许多属于通常与杀虫剂抗性无关的家族的基因，如肽酶，钠/钙交换剂和涉及脂质和碳水化合物代谢的基因。还检测了拟除虫菊酯抗性RSP菌株暴露于氯菊酯的短期（6和10 h）影响。几个基因属于酶的家庭已经牵连在杀虫剂或异生物质解毒诱导，包括羧酸酯酶COEAE 2F基因和UDP-葡萄糖醛酸转移酶和腈水解酶家族的成员。
A microarray containing approximately 20 000 expressed sequence tags (ESTs; 11 760 unique EST clusters) from the malaria vector, Anopheles gambiae, was used to monitor differences in global gene expression in two insecticide resistant and one susceptible strains. Statistical analysis identified 77 ESTs that were differentially transcribed among the three strains. These include the cytochrome P450 CYP314A1, over-transcribed in the DDT resistant ZAN/U strain, and many genes that belong to families not usually associated with insecticide resistance, such as peptidases, sodium/calcium exchangers and genes implicated in lipid and carbohydrate metabolism. Short-term (6 and 10 h) effects of exposure of the pyrethroid resistant RSP strain to permethrin were also detected. Several genes belonging to enzyme families already implicated in insecticide or xenobiotic detoxification were induced, including the carboxylesterase COEAE2F gene and members of the UDP-glucuronosyl transferase and nitrilase families.