Human retinal microglia: Expression of immune markers and relationship to the Glia limitans
Human retinal microglia: Expression of immune markers and relationship to the Glia limitans
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DOI：
10.1002/glia.440140402
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发表时间：
1995-08
期刊：
影响因子：
6.2
通讯作者：
J. Provis;P. Penfold;Antony J. Edwards;D. van Driel
中科院分区：
文献类型：
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作者：
J. Provis;P. Penfold;Antony J. Edwards;D. van Driel
使用免疫金组织化学、电子显微镜 (EM) 和针对 CD45、主要组织相容性复合物 I 类 (MHC-I)、MHC-II 和人巨噬细胞抗原的抗体，在正常人视网膜的平片和切片中研究小胶质细胞的免疫反应性、形态及其与胶质细胞界限的关系。所有测试的抗体都具有明显的免疫反应性，包括标记小胶质细胞和视网膜血管内皮的 MHC-I。使用 CD45、MHC-II 和抗人巨噬细胞 (S22) 抗原的抗体获得了最一致的标记。在血管周围空间（血管周围细胞）和视网膜实质（小胶质细胞）中观察到免疫反应性细胞，它们与血管轮廓紧密贴壁。一些实质小胶质细胞也与血管相关，并且通过电镜观察发现其与神经胶质限制细胞（血管旁小胶质细胞）密切相关。光密度测定显示，血管旁小胶质细胞比邻近的非血管相关实质小胶质细胞表达更高水平的 MHC 抗原。此外，血管旁小胶质细胞巨噬细胞（S22）抗原阳性，而其他实质小胶质细胞不表达巨噬细胞抗原。定量数据表明，相似的小胶质细胞群体对 CD45、MHC-I 和 MHC-II 具有免疫反应性，而相对较少的小胶质细胞（约 10%）对人巨噬细胞 (S22) 抗原具有免疫反应性，这支持了之前关于小胶质细胞是异质群体的观点。 © 1995 Wiley-Liss, Inc.
The immunoreactivity, morphology and relationship to the glia limitans of microglia were investigated in flatmounts and sections of normal human retina, using immunogold histochemistry, electron microscopy (EM), and antibodies directed against CD45, major histocompatability complex class I (MHC‐I), MHC‐II, and human macrophage antigens. Immunoreactivity was evident for all antibodies tested, including MHC‐I, which labeled both microglia and retinal vascular endothelium. Most consistent labeling was obtained using antibodies to CD45, MHC‐II, and anti‐human macrophage (S22) antigen. Immunoreactive cells were seen in the perivascular space (perivascular cells), where they were closely adherent to the vessel profile, and in the retinal parenchyma (microglia). Some parenchymal microglia were also vessel associated and by EM were seen to be closely related to the glia limitans (paravascular microglia). Paravascular microglia were shown by optical densitometry, to express higher levels of MHC antigens than neighboring, non‐vessel associated, parenchymal microglia. In addition, paravascular microglia were macrophage (S22) antigen positive, while other parenchymal microglia did not express macrophage antigens. Quantitative data indicate that similar populations of microglia are immunoreactive to CD45, MHC‐I, and MHC‐II, while relatively few microglia (approximately 10%) are immunoreactive for human macrophage (S22) antigens, supporting previous suggestions that microglia are a heterogeneous population. © 1995 Wiley‐Liss, Inc.