Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interface
Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interface
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DOI：
10.1016/j.intimp.2021.107457
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发表时间：
2021-03-20
影响因子：
5.6
通讯作者：
Portoles, M. T.
中科院分区：
文献类型：
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作者：
Diez-Orejas, R.;Casarrubios, L.;Portoles, M. T.
使用纳米颗粒进行细胞内药物递送可以降低药物的毒性和副作用，但是这些纳米载体的摄取在它们掺入后会对细胞和组织产生不良影响。巨噬细胞在宿主防御中发挥核心作用，并负责体内纳米颗粒运输。评估纳米颗粒吸收后它们对病原微生物的防御能力，对于预防与纳米颗粒治疗相关的感染是必要的。在这项研究中，中空介孔SiO2 CaO纳米球标记的异硫氰酸荧光素（FITC-NanoMBGs）对腹腔巨噬细胞的功能的影响进行了评估，通过测量其吞噬表达红色荧光蛋白的白色念珠菌的能力。使用两种巨噬细胞/真菌比率（MOI 1和MOI 5），并进行两种实验策略：a）用FITC-NanoMBG预处理巨噬细胞并随后进行真菌感染; B）同时添加真菌和纳米球后的竞争测定。还评价了巨噬细胞促炎表型标志物（CD 80表达和白细胞介素6分泌）。30 min后观察到CD 80+巨噬细胞百分比和白细胞介素6分泌的显著降低，表明NanoMBG和真菌的同时掺入有利于巨噬细胞非炎性表型。本研究证明，在所有研究条件下摄取这些纳米球不会改变巨噬细胞功能。此外，细胞内FITC-NanoMBG在MOI 1和短时间的相互作用后诱导巨噬细胞的真菌吞噬作用的暂时增加。在竞争试验中，随着细胞内真菌数量的增加，细胞内FITC-NanoMBG含量以MOI和时间依赖性方式降低。这些结果已经证实，巨噬细胞在动态细胞内掺入中清楚地区分惰性材料和活酵母。此外，巨噬细胞吞噬作用是了解其功能状态的关键决定因素，也是研究纳米材料/巨噬细胞/白色念珠菌界面的有价值参数。
The use of nanoparticles for intracellular drug delivery could reduce the toxicity and side effects of the drug but, the uptake of these nanocarriers could induce adverse effects on cells and tissues after their incorporation. Macrophages play a central role in host defense and are responsible for in vivo nanoparticle trafficking. Assessment of their defense capacity against pathogenic micro-organisms after nanoparticle uptake, is necessary to prevent infections associated with nanoparticle therapies. In this study, the effects of hollow mesoporous SiO2CaO nanospheres labeled with fluorescein isothiocyanate (FITC-NanoMBGs) on the function of peritoneal macrophages was assessed by measuring their ability to phagocytize Candida albicans expressing a red fluorescent protein. Two macrophage/fungus ratios (MOI 1 and MOI 5) were used and two experimental strategies were carried out: a) pretreatment of macrophages with FITC-NanoMBGs and subsequent fungal infection; b) competition assays after simultaneous addition of fungus and nanospheres. Macrophage pro-inflammatory phenotype markers (CD80 expression and interleukin 6 secretion) were also evaluated. Significant decreases of CD80+ macrophage percentage and interleukin 6 secretion were observed after 30 min, indicating that the simultaneous incorporation of NanoMBG and fungus favors the macrophage non-inflammatory phenotype. The present study evidences that the uptake of these nanospheres in all the studied conditions does not alter the macrophage function. Moreover, intracellular FITC-NanoMBGs induce a transitory increase of the fungal phagocytosis by macrophages at MOI 1 and after a short time of interaction. In the competition assays, as the intracellular fungus quantity increased, the intracellular FITC-NanoMBG content decreased in a MOI- and time-dependent manner. These results have confirmed that macrophages clearly distinguish between inert material and the live yeast in a dynamic intracellular incorporation. Furthermore, macrophage phagocytosis is a critical determinant to know their functional state and a valuable parameter to study the nanomaterial / macrophages / Candida albicans interface.