Formation of 2-Hydroxydeoxyadenosine Triphosphate, an Oxidatively Damaged Nucleotide, and Its Incorporation by DNA Polymerases
Formation of 2-Hydroxydeoxyadenosine Triphosphate, an Oxidatively Damaged Nucleotide, and Its Incorporation by DNA Polymerases
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2-羟基脱氧腺苷三磷酸（一种氧化损伤的核苷酸）的形成及其通过 DNA 聚合酶的掺入
DOI：
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发表时间：
1995
影响因子：
4.8
通讯作者：
H. Kasai
中科院分区：
文献类型：
--
作者：
H. Kamiya;H. Kasai
We found that hydroxylation occurs at the C-2 position of adenine by oxygen radical treatment (Fe-EDTA) of dA, dATP, and single- and double-stranded DNA. This oxidatively damaged base, 2-hydroxyadenine, was produced 3-6-fold and 40-fold less than 8-hydroxyguanine when monomers and polynucleotides, respectively, were treated. To determine whether the damaged nucleotide, 2-hydroxydeoxyadenosine triphosphate (2-OH-dATP), is incorporated into a growing DNA, and to reveal the kinds of nucleotides opposite which 2-OH-dATP is incorporated, calf thymus DNA polymerase α and the Klenow fragment of Escherichia coli DNA polymerase I were used in in vitro DNA synthesis in the presence of 2-OH-dATP. DNA polymerase α incorporated the nucleotide opposite T and C in the DNA template. On the other hand, in an experiment using the Klenow fragment, incorporation of 2-OH-dATP was observed only opposite T. Steady-state kinetic studies indicated that incorporation of 2-OH-dATP by DNA polymerase α opposite T was favored over that opposite C by a factor of only 4.5. These results indicate that 2-OH-dATP, an oxidatively damaged nucleotide, is a substrate for DNA polymerases and is incorporated incorrectly by the replicative DNA polymerase.
影响因子：
14.9
作者：
PURMAL, AA;KOW, YW;WALLACE, SS
通讯作者：
WALLACE, SS
影响因子：
2.9
作者：
WOOD, ML;DIZDAROGLU, M;ESSIGMANN, JM
通讯作者：
ESSIGMANN, JM
最近邻对 DNA 聚合酶插入保真度的影响。
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Mendelman,LV;Boosalis,MS;Petruska,J;Goodman,MF
通讯作者：
Goodman,MF