Effect of Organic Selenium Supplementation on Selenium Status, Oxidative Stress, and Antioxidant Status in Selenium-Adequate Dairy Cows During the Periparturient Period
Effect of Organic Selenium Supplementation on Selenium Status, Oxidative Stress, and Antioxidant Status in Selenium-Adequate Dairy Cows During the Periparturient Period
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补充有机硒对足硒奶牛围产期硒状态、氧化应激和抗氧化状态的影响
DOI：
10.1007/s12011-018-1323-0
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发表时间：
2018-12-01
影响因子：
3.9
通讯作者：
Xiao, Min
中科院分区：
文献类型：
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作者：
Gong, Jian;Xiao, Min
围产期是奶牛从妊娠晚期向泌乳早期过渡的紧张时期。氧化应激在此期间由于代谢活动增加而发生。补充略高于建议要求的抗氧化剂可能有助于缓解这种压力。本研究的目的是确定是否补充硒（Se）酵母日粮中有足够的硒浓度影响硒状态，氧化应激和抗氧化状态的奶牛在围产期。将20头经产荷斯坦奶牛随机分为两组，每组10个重复。在产犊前的最后4周期间，奶牛除了在其口粮中以0.3 mg Se/kg干物质的亚硒酸钠外，还以0（对照）或0.3 mg Se/kg干物质的Se-酵母（Se-酵母补充剂）饲喂。测定了奶牛产后21和7日龄血浆和红细胞中硒、活性氧（ROS）、过氧化氢（H2 O2）、羟自由基、丙二醛（MDA）、α-生育酚（α-tocopherol）和谷胱甘肽（GSH）的含量、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性以及总抗氧化能力（T-AOC）。产后第7天和第21天。与对照组相比，在妊娠最后4周添加硒酵母的奶牛在产后7 d、产后7 d和产后21 d血浆硒水平较高，MDA水平较低，全血硒水平较高，血浆ROS和H2 O2水平较低。硒酵母补充增加血浆和红细胞GSH-Px活性和红细胞GSH浓度在产后7天相比，硒充足的控制奶牛。与对照组相比，添加硒酵母的奶牛产后7天和21天血浆中SOD和CAT活性提高，α-生育酚和GSH含量增加，T-AOC含量增加。结果表明，在妊娠后期喂饲硒充足的奶牛，添加硒酵母可提高血浆硒水平，改善机体抗氧化功能，有效缓解泌乳早期的氧化应激。
The periparturient period represents a stressful time for dairy cows as they transition from late gestation to early lactation. Oxidation stress occurs during this period owing to the increased metabolic activity. Antioxidants supplementation slightly above the suggested requirements may be beneficial in relieving this kind of stress. The objective of this study was to determine whether supplementing selenium (Se) yeast to diets with adequate Se concentrations affects Se status, oxidative stress, and antioxidant status in dairy cows during the periparturient period. Twenty multiparous Holstein cows were randomly divided into two groups with ten replicates in each group. During the last 4 weeks before calving, cows were fed Se-yeast at 0 (control) or 0.3 mg Se/kg dry matter (Se-yeast supplementation), in addition to Na selenite at 0.3 mg Se/kg dry matter in their rations. The concentrations of Se, reactive oxygen species (ROS), hydrogen peroxide (H2O2), hydroxyl radical, malonaldehyde (MDA), α-tocopherol and glutathione (GSH), the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT), and the total antioxidant capacity (T-AOC) in plasma or erythrocyte of dairy cows were measured at 21 and 7 days prepartum, and at 7 and 21 days postpartum. Cows fed Se-yeast supplement during the last 4 weeks of gestation had higher plasma Se and lower MDA concentrations at 7 days prepartum, and at 7 and 21 days postpartum, and had higher whole blood Se and lower plasma ROS and H2O2concentrations at 7 and 21 days postpartum compared with control cows. Se-yeast supplementation increased plasma and erythrocyte GSH-Px activities and erythrocyte GSH concentration at 7 days postpartum as compared to Se-adequate control cows. Compared with control cows, the enhanced SOD and CAT activities, increased α-tocopherol and GSH concentrations, and improved T-AOC in plasma at 7 and 21 days postpartum in Se-yeast-supplemented cows were also observed in this study. The results indicate that feeding Se-adequate cows a Se-yeast supplement during late gestation increases plasma Se status, improves antioxidant function, and relieves effectively oxidative stress occurred in early lactation.