Liquid-based cervical cytology ThinPrep screening in Hungary
Liquid-based cervical cytology ThinPrep screening in Hungary
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DOI：
10.1556/oh.2014.29875
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发表时间：
2014-05-01
期刊：
影响因子：
0.6
通讯作者：
Szentirmay Zoltan
中科院分区：
文献类型：
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作者：
Bak Mihaly;Seberne Ell Maria;Szentirmay Zoltan
介绍：液基宫颈细胞学检查已发展成为传统巴氏宫颈细胞学检查的替代方法。目的：探讨宫颈液基细胞学检查的质量保证。方法：对4573例宫颈细胞学涂片按Bethesda（2001）分类法进行分类。人乳头瘤病毒感染检测和亚型反射试验，使用实时聚合酶链反应。结果：4573例涂片按Bethesda（2001）分类系统分类。阴性诊断2323例（50.8%），非肿瘤性2017例（44.1%），上皮内病变或恶性233例（5.1%）。涂片诊断不满意43例（0.9%），低度鳞状上皮内病变87例（1.9%），高度鳞状上皮内病变24例（0.5%），癌23例（0.5%）。其中59例经组织学证实，4例假阴性。其敏感性、特异性和阳性预测值分别为93.2%、100%和100%。与前三年相同的时间段相比，低级别和高级别鳞状上皮内病变从0.82%增加到2.51%。81例人乳头瘤病毒检测阳性预测值为99%。结论：液基宫颈细胞学检查结果的审核值符合建议的阈值。液基宫颈细胞学检查是一种可供选择的宫颈细胞学检查方法，在所有参数方面似乎明显优于传统的巴氏宫颈细胞学检查。
Introduction: Liquid-based cervical cytology has been developed as an alternative for conventional Papanicolaou cervical cytology. Aim: The aim of this study was to determine the quality assurance of liquid-based cervical cytology. Method: 4573 cervical cytology smears were classified according to the Bethesda (2001) system. Human papilloma virus infection was detected and subtyped from reflex test using real-time polymerase chain reaction. Results: 4573 smears were classified according to the Bethesda (2001) system. Negative diagnosis was made in 2323 cases (50.8%), non neoplastic in 2017 cases (44.1%), and positive for intraepithelial lesions or malignancy in 233 cases (5.1%). Unsatisfactory smear for diagnosis was found in 43 cases (0.9%), low-grade squamous intraepithelial lesion in 87 cases (1.9%), high-grade squamous intraepithelial lesion in 24 cases (0.5%), and carcinoma in 23 cases (0.5%). Fifty-nine of the cases were histologically verified and 4 falsely negative cases were detected. The sensitivity, specificity and the positive predictive value were 93.2%, 100% and 100%, respectively. Compared to an identical time periods of the previous three years, the low-and high-grade squamous intraepithelial lesion increased from 0.82% to 2.51%. Eighty one human papilloma virus tests were performed with a positive predictive value of 99%. Conclusions: The auditing values of the liquid-bases cervical cytology results meet the proposed threshold values. Liquid-bases cervical cytology is an alternative cervical cytology and it seems to be significantly better than conventional Papanicolaou cervical cytology in all parameters.