Rapid detection of Candida species in bronchoalveolar lavage fluid from patients with pulmonary symptoms.
Rapid detection of Candida species in bronchoalveolar lavage fluid from patients with pulmonary symptoms.
复制标题
DOI：
10.1016/j.bjm.2015.02.001
复制
发表时间：
2016-01
期刊：
影响因子：
--
通讯作者：
Mohammadi R
中科院分区：
文献类型：
--
作者：
Zarrinfar H;Kaboli S;Dolatabadi S;Mohammadi R
念珠菌属物种，尤其是白色念珠菌，是人类粘膜表面的共生菌，但从其在免疫功能低下患者和抗生素消耗中的患病率上升可见，它正日益成为重要的侵袭性病原体之一。因此，准确鉴定有肺部症状的患者中的念珠菌种类可以为有效治疗提供重要信息。从有肺部症状的免疫功能低下和免疫功能正常患者的支气管肺泡灌洗液中获得了总共 75 种念珠菌临床分离株。通过聚合酶链式反应-限制性片段长度多态性 (PCR-RFLP) 进行核核糖体内转录间隔区 (ITS1-ITS2 rDNA) 序列分析来鉴定念珠菌培养物。分子鉴定表明，分离株主要属于白色念珠菌（52%），其次是热带念珠菌（24%）、光滑念珠菌（14.7%）、克柔念珠菌（5.3%）、近平滑念珠菌（1.3%）、克菲尔念珠菌（1.3%）和吉里蒙念珠菌（1.3%）。鉴于不同患者的疾病概况及其治疗方案日益复杂，快速、准确地识别念珠菌种类可以导致及时、适当的抗真菌治疗。
Candida species, especially C. albicans, are commensals on human mucosal surfaces, but are increasingly becoming one of the important invasive pathogens as seen by a rise in its prevalence in immunocompromised patients and in antibiotic consumption. Thus, an accurate identification of Candida species in patients with pulmonary symptoms can provide important information for effective treatment. A total of 75 clinical isolates of Candida species were obtained from the bronchoalveolar lavage fluid of both immunocompromised and immunocompetent patients with pulmonary symptoms. Candida cultures were identified based on nuclear ribosomal Internal Transcribed Spacer (ITS1-ITS2 rDNA) sequence analysis by polymerase chain reaction–restriction fragment length polymorphisms (PCR-RFLP). Molecular identification indicated that the isolates belonged predominantly to C. albicans (52%), followed by C. tropicalis (24%), C. glabrata (14.7%), C. krusei (5.3%), C. parapsilosis (1.3%), C. kefyr (1.3%) and C. guilliermondii (1.3%). Given the increasing complexity of disease profiles and their management regimens in diverse patients, rapid and accurate identification of Candida species can lead to timely and appropriate antifungal therapy.