Characterizing the spatial and temporal occurrence patterns of the endangered botiid loach Parabotia curtus by environmental DNA analysis using a newly developed species-specific primer set.
Characterizing the spatial and temporal occurrence patterns of the endangered botiid loach Parabotia curtus by environmental DNA analysis using a newly developed species-specific primer set.
复制标题
使用新开发的物种特异性引物组进行环境 DNA 分析，表征濒临灭绝的 botidid 泥鳅 Parabotia curtus 的空间和时间发生模式。
DOI：
10.1007/s10228-020-00756-4
复制
发表时间：
2021
影响因子：
1.2
通讯作者：
Watanabe K
中科院分区：
文献类型：
--
作者：
Sugiura K;Tomita S;Minamoto T;Mishina T;Iwata A;Abe T;Yamamoto S;Watanabe K
本文报道了一种用于检测环境沃茨中濒危泥鳅Parabotia curtus的特异性引物。利用从黑腹叶蜂组织中提取的DNA进行引物试验。curtus等4种泥鳅对P. curtus引物集的应用，在一个自然的栖息地，全年收集的实地样品证实了它的能力，这种罕见的，小鱼，往往居住在浑浊的沃茨的空间和时间的发生模式的特点。
We report a novel species-specific primer set for detection of the endangered botiid loachParabotia curtusin environmental waters. A primer test using DNA extracted from tissues ofP. curtusand four other loach species indicated its high specificity toP. curtus. Application of the primer set to field samples collected throughout the year in a natural habitat confirmed its ability to characterize spatial and temporal occurrence patterns of this rare, small fish that often inhabits turbid waters.