Acetamiprid, a neonicotinoid insecticide, induced cytotoxicity and genotoxicity in PC12 cells
Acetamiprid, a neonicotinoid insecticide, induced cytotoxicity and genotoxicity in PC12 cells
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DOI：
10.1080/15376516.2019.1624907
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发表时间：
2019-10-13
影响因子：
3.2
通讯作者：
Abid-Essefi, Salwa
中科院分区：
文献类型：
--
作者：
Annabi, Emna;Ben Salem, Intidhar;Abid-Essefi, Salwa
新烟碱类是一组广泛用于农业和家庭的杀虫剂。在这些除害剂中，啶虫脒（acetamiprid，ACM）是一种广谱的除害剂，用于保护蔬菜和水果免受虫害。这种农药的广泛使用导致了环境污染，包括土壤，水以及食品。然而，关于ACM发挥其细胞毒性和遗传毒性作用的分子机制的信息很少。本研究的目的是研究ACM对PC 12细胞的毒性作用。我们证明，ACM显着降低细胞活力的MTT法评估。我们还表明，ACM诱导的活性氧（ROS）的产生，随后通过MDA水平的增加证明脂质过氧化。细胞死亡的增加伴随着线粒体膜电位的降低。此外，Z-VAD-FMK，一个通用的半胱氨酸蛋白酶抑制剂预处理，显着减少ACM诱导的细胞死亡。我们的研究结果还表明，ACM诱导的DNA损伤的浓度依赖性增加明显的彗星试验。这些数据表明，ACM在哺乳动物细胞中产生细胞毒性和DNA损伤。
Neonicotinoids are a group of pesticides widely used in agriculture and at home. Among those pesticides, acetamiprid (ACM) is a broad-spectrum insecticide used for the protection of vegetables and fruits from pest. The extensive use of this pesticide had led to contamination of environment including soil, water, as well as food products. However, there are few informations regarding the molecular mechanism by which ACM exerts its cytotoxic and genotoxic effects. The aim of the present study was to investigate the toxic effects of ACM in PC12 cells. We demonstrated that ACM significantly decreased cell viability as assessed by the MTT assay. We also shown that ACM-induced reactive oxygen species (ROS) generation followed by lipid peroxidation as evidenced by an increase in the MDA levels. The increase in cell death was accompanied by a reduction in the mitochondrial membrane potential. Besides, pretreatment with Z-VAD-FMK, a general caspases inhibitor, significantly decreased the ACM-induced cell death. Our results also indicate that ACM induced a concentration-dependent increase in DNA damage as evident by the Comet assay. These data indicate that ACM produces cytotoxicity and DNA damage in mammalian cells.