Relationship of membrane physical properties to alcohol dependence in mice selected for genetic differences in alcohol withdrawal.
Relationship of membrane physical properties to alcohol dependence in mice selected for genetic differences in alcohol withdrawal.
复制标题
因酒精戒断遗传差异而选择的小鼠的膜物理特性与酒精依赖的关系。
DOI：
10.1016/0024-3205(84)90027-4
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发表时间：
1984
期刊：
影响因子：
6.1
通讯作者：
McSwigan,JD
中科院分区：
文献类型：
--
作者：
Harris,RA;Crabbe,JC;McSwigan,JD
基于吸入乙醇蒸汽后戒断发作严重程度的遗传选择产生了两种小鼠品系，WSR（戒断发作抵抗）和WSP（戒断发作倾向），它们在戒断体征上明显不同。在本研究中，我们报告说，这些小鼠也不同的严重程度，戒断发作后，消费含乙醇的液体饮食，但没有不同的乙醇摄入量。与乙醇戒断发作相反，这些线对电或戊四唑诱导的发作显示出相似的敏感性。这些结果表明，线不同的发展，身体依赖乙醇，而不是癫痫敏感性本身。由于突触膜流动性降低与乙醇依赖性有关，我们使用二苯基己三烯和三甲基铵-二苯基己三烯的荧光偏振来评估喂食实验室食物、含乙醇的液体饮食或含等热量蔗糖的液体饮食的WSP和WSR小鼠的膜流动性。对于喂食实验室食物的WSP和WSR小鼠，脑突触膜的流动性相同。对照流质饮食没有改变膜流动性，乙醇饮食同样降低了WSP和WSR小鼠的流动性。因此，在这些品系中发现的乙醇依赖性发展的遗传差异并没有反映在脑膜的物理性质中。
Genetic selection based on severity of withdrawal seizures following inhalation of ethanol vapor has produced two lines of mice, WSR (withdrawal seizure resistant) and WSP (withdrawal seizure prone), that differ markedly in withdrawal signs. In the present study, we report that these mice also differed in the severity of withdrawal seizures following consumption of an ethanol-containing liquid diet but did not differ in ethanol intake. In contrast to ethanol withdrawal seizures, the lines displayed similar sensitivity to electrical-or pentylenetetrazole-induced seizures. These results suggest that the lines differ in the development of physical dependence on ethanol rather than seizure sensitivity per se. Because decreased synaptic membrane fluidity has been associated with ethanol dependence, we used fluorescence polarization of diphenylhexatriene and trimethylammonium-diphenylhexatriene to evaluate membrane fluidity in WSP and WSR mice fed lab chow, an ethanol-containing liquid diet, or an isocaloric sucrose-containing liquid diet. Fluidity of brain synaptic membranes was identical for WSP and WSR mice fed lab chow. The control liquid diet did not alter membrane fluidity, and the ethanol diet decreased fluidity equally for WSP and WSR mice. Thus, the genetic difference in development of ethanol dependence found in these lines was not reflected in the physical properties of brain membranes.