Cellular mechanisms of callose deposition in response to fungal infection or chemical damage
Cellular mechanisms of callose deposition in response to fungal infection or chemical damage
复制标题
响应真菌感染或化学损伤的胼胝质沉积的细胞机制
作者：
D. Škalamera;M. C. Heath
用化学抑制剂研究了豇豆锈菌、硼酸或多抗霉素D杀死的锈菌诱导豇豆叶片胼胝质沉积的细胞机制。抑制剂的影响，观察在这两个电阻和敏感豇豆品种。抑制剂的效果不同，这取决于胼胝体诱导刺激的类型和品种使用。转录抑制剂（放线菌素D）和蛋白质合成（杀稻瘟菌素S，放线菌酮）降低真菌诱导的胼胝质沉积在两个品种的发病率。蛋白质合成的抑制剂也减少了硼酸或真菌死亡引起的沉积。蛋白质糖基化抑制剂（衣霉素、脱氧野尻霉素）和微丝功能抑制剂（细胞松弛素B和E）能抑制抗病品种中活菌诱导的胼胝质沉积，但对感病品种中胼胝质沉积和化学或真菌诱导的胼胝质沉积无影响。
Cellular mechanisms of callose deposition induced in cowpea (Vigna unguiculata) leaves by the living cowpea rust fungus (Uromyces vignae), boric acid, or the fungus killed by polyoxin D, were investigated by the use of chemical inhibitors. Effects of the inhibitors were observed in both a resistant and a susceptible cowpea cultivar. The effect of inhibitors differed depending on the type of callose-inducing stimulus and cultivar used. Inhibitors of transcription (actinomycin D) and protein synthesis (blasticidin S, cycloheximide) lowered the incidence of fungus-induced callose deposits in both cultivars. Inhibitors of protein synthesis also reduced deposits induced by boric acid or fungal death. Callose deposition induced by the living fungus in the resistant cultivar was reduced by inhibitors of protein glycosylation (tunicamycin, deoxynojirimycin) and microfilament function (cytochalasins B and E), but these inhibitors had no effect on callose deposition in the susceptible cultivar or on chemical or fun...