A genome sequence survey of the mollicute corn stunt spiroplasma Spiroplasma kunkelii
A genome sequence survey of the mollicute corn stunt spiroplasma Spiroplasma kunkelii
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DOI：
10.1111/j.1574-6968.2002.tb11153.x
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发表时间：
2002-04-23
影响因子：
2.1
通讯作者：
Hogenhout, SA
中科院分区：
文献类型：
--
作者：
Bai, XD;Hogenhout, SA
玉米矮化螺原体（Spiroplasma kunkelii）是一种由玉米叶蝉传播的病原菌。对S. kunkelii的启动是为了帮助理解螺原体与其植物和叶蝉宿主相互作用的遗传基础。总共获得了144712个非冗余的高质量S。获得kunkelii基因组序列，在支原体科和芽孢杆菌/梭菌数据库中搜索序列标签。结果表明，除了螺原体噬菌体SpV 1 DNA插入，螺原体基因组具有更多的嘌呤和氨基酸的生物合成。转录调控、细胞被膜和DNA转运/结合基因。这项研究表明，调查测序是一个有效的程序，基因发现和基因组表征。S. kunkelii测序项目可在Spiroplasma网页http://www.oardc.ohio-state.edu/spiroplasma/genome.htm上获得。(C)2002年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
The mollicute corn stunt spiroplasma (Spiroplasma kunkelii) is a leafhopper-transmitted pathogen of maize. Sequencing of the similar to1.6-Mb genome of S. kunkelii was initiated to aid understanding the genetic basis of spiroplasma interactions with their plant and leafhopper hosts. In total, 144 712 nucleotides of non-redundant, high-quality S. kunkelii genome sequence were obtained, Sequence tags were searched against the Mycoplasmataceae and Bacillus/Clostridium databases. Results showed that, in addition to spiroplasma phage SpV1 DNA insertions, spiroplasma genomes harbor more purine and amino acid biosynthesis. transcription regulation, cell envelope and DNA transport/binding genes than Mycoplasmataceae genomes. This investigation demonstrates that survey sequencing is an efficient procedure for gene discovery and genome characterization. The results of the S. kunkelii sequencing project are available at the Spiroplasma WebPage at http://www.oardc.ohio-state.edu/spiroplasma/genome.htm. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.