Protein/Polymer-Based Dual-Responsive Gold Nanoparticles with pH-Dependent Thermal Sensitivity
Protein/Polymer-Based Dual-Responsive Gold Nanoparticles with pH-Dependent Thermal Sensitivity
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DOI：
10.1002/adfm.201102471
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发表时间：
2012-04-10
影响因子：
19
通讯作者：
Liz-Marzan, Luis M.
中科院分区：
文献类型：
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作者：
Strozyk, Malte S.;Chanana, Munish;Liz-Marzan, Luis M.
本文介绍了基于表面引发原子转移自由基聚合（SI-ATRP）的蛋白质包覆的金纳米粒子接枝热敏聚合物刷的具有可逆光学性质的双响应等离子体纳米粒子的合成和物理化学行为，其表现出pH依赖的温度响应行为。首先用牛血清白蛋白（BSA）将两种不同尺寸（15 nm和60 nm）和具有不同稳定剂（分别为柠檬酸盐和十六烷基三甲基溴化铵（CTAB））的球形金纳米颗粒封端。所得到的BSA-封端的NPs（Au@BSA NPs）不仅在生理条件下表现出极高的胶体稳定性，而且具有与纯BSA相似的可逆U形pH响应行为。什么？-然后使用蛋白质涂层中的L-赖氨酸的胺共价结合ATRP引发剂，允许低聚（乙二醇）甲基丙烯酸酯的热敏聚合物刷的SI-ATRP，其在纯水中的LCST为42 ℃，在生理条件下为约37 ℃。这种接枝有热敏聚合物的蛋白质包被的纳米颗粒表现出智能的pH依赖性热敏行为。
This article presents the synthesis and physicochemical behavior of dual-responsive plasmonic nanoparticles with reversible optical properties based on protein-coated gold nanoparticles grafted with thermosensitive polymer brushes by means of surface-initiated atom transfer radical polymerization (SI-ATRP) that exhibit pH-dependent thermo-responsive behavior. Spherical gold NPs of two different sizes (15 nm and 60 nm) and with different stabilizing agents (citrate and cetyltrimethylammonium bromide (CTAB), respectively) were first capped with bovine serum albumin (BSA). The resulting BSA-capped NPs (Au@BSA NPs) exhibited not only extremely high colloidal stability under physiological conditions, but also a reversible U-shaped pH-responsive behavior, similar to pure BSA. The ?-amine of the L-lysine in the protein coating was then used to covalently bind an ATRP-initiator, allowing for the SI-ATRP of thermosensitive polymer brushes of oligo(ethylene glycol) methacrylates with an LCST of 42 degrees C in pure water and around 37 degrees C under physiological conditions. Such protein coated nanoparticles grafted with thermosensitive polymers exhibit a smart pH-dependent thermosensitive behavior.