Surface energy of hydroxyapatite and ß-tricalcium phosphate ceramics driving serum protein adsorption and osteoblast adhesion
Surface energy of hydroxyapatite and ß-tricalcium phosphate ceramics driving serum protein adsorption and osteoblast adhesion
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DOI：
10.1007/s10856-007-3347-4
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发表时间：
2008-06-01
影响因子：
3.7
通讯作者：
Anselme, K.
中科院分区：
文献类型：
--
作者：
dos Santos, E. A.;Farina, M.;Anselme, K.
这项工作的主要目的是评估磷酸钙表面能对血清蛋白吸附和人成骨细胞粘附的具体作用，通过隔离化学效应所造成的地形。高密度磷酸盐陶瓷（单相羟基磷灰石HA和β-磷酸三钙β-TCP）呈现两种不同的纳米粗糙度。一些样品被金溅射涂层，以方便地掩盖表面化学效应（不修改原始粗糙度），并研究表面形貌对细胞行为的孤立影响。结果表明，磷酸钙的纳米形貌对蛋白质吸附过程的影响比表面化学更重要。成骨细胞的接种功效既不受地形的影响，也不受磷酸钙化学物质的影响，但β-TCP化学物质对细胞扩散有负面影响。我们观察到，表面疏水性是改变蛋白质吸附在表面上的另一种方式。表面能的极性分量的减少导致了减少的白蛋白和纤连蛋白的吸附，但增加细胞粘附。总体而言，这项工作有助于更好地了解磷酸钙在血清蛋白吸附和成骨细胞粘附中的地形和表面化学作用。
The main objective of this work was to evaluate the specific role of calcium phosphates surface energy on serum protein adsorption and human osteoblast adhesion, by isolating chemical effects from those caused by topography. Highly dense phosphate ceramics (single-phase hydroxyapatite HA and beta-tricalcium phosphates beta-TCP) presenting two distinct nano roughnesses were produced. Some samples were gold-sputter coated in order to conveniently mask the surface chemical effects (without modification of the original roughness) and to study the isolated effect of surface topography on cellular behavior. The results indicated that the nano topography of calcium phosphates strongly affected the protein adsorption process, being more important than surface chemistry. The seeding efficacy of osteoblasts was not affected nor by the topography neither by the calcium phosphate chemistries but the beta-TCP chemistry negatively influenced cell spreading. We observed that surface hydrophobicity is another way to change protein adsorption on surfaces. The decrease of the polar component of surface energy on gold-coated samples leaded to a decreased albumin and fibronectin adsorption but to an increased cell adhesion. Overall, this work contributes to better understand the role of topography and surface chemistry of calcium phosphates in serum protein adsorption and osteoblast adhesion.