THE ENZYMES OF POLYPHOSPHATE METABOLISM IN VESICULAR‐ARBUSCULAR MYCORRHIZAS
THE ENZYMES OF POLYPHOSPHATE METABOLISM IN VESICULAR‐ARBUSCULAR MYCORRHIZAS
复制标题
囊泡-丛枝菌根中多磷酸盐代谢酶
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
J. Callow
中科院分区：
文献类型：
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作者：
L. Capaccio;J. Callow
总结
研究了洋葱菌根和非菌根根以及内生真菌Glomus mosseae的外部和内部菌丝中聚磷酸盐（polyP）合成和降解酶的存在和分布。PolyP激酶活性检测外菌丝和菌根根提取物。活性依赖于外源聚磷和镁离子，反应产物是一个长链聚磷。外聚磷酸酶活性检测在菌根和非菌根根，但活性是134°O在前者更大。内生多磷酸酶活性也更大的菌根根。多聚磷酸酶检测内部菌丝提取物，但没有外部菌丝。在外部菌丝和菌根根的提取物中证明了多磷酸葡萄糖激酶活性，直接反应产物是葡萄糖-6-磷酸。这些结果进行了讨论，在有关的潜在机制，磷转运和转移泡状丛枝（VA）菌根。报道了一种从洋葱菌根根中分离内部菌丝、囊泡和丛枝的新技术。
SUMMARY
The presence and distribution of enzymes of polyphosphate (polyP) synthesis and degrActation was examined in mycorrhizal and non-mycorrhizal onion roots, and both external and internal hyphae of the endophyte, Glomus mosseae. PolyP kinase activity was detected in extracts of external hyphae and mycorrhizal roots. Activity was dependent on exogenous polyP and Mg2+, the reaction product being a long chain polyP. Exopolyphosphatase activity was detected in both mycorrhizal and non-mycorrhizal roots but activity was 134°O greater in the former. Endopolyphosphatase activity was also greater in mycorrhizal roots. Polyphosphatases were detected in extracts of internal hyphae, but not external hyphae. Polyphosphate glucokinase activity was demonstrated in extracts of external hyphae and mycorrhizal roots, the immediate reaction product being glucose-6-phosphate. These results are discussed in relation to potential mechanisms of P translocation and transfer in vesicular-arbuscular (VA) mycorrhizas. A novel technique for isolating the internal hyphae and associated vesicles and arbuscules from mycorrhizal onion roots is described.