Spatial and temporal distribution of nerves, ganglia, and smooth muscle during the early pseudoglandular stage of fetal mouse lung development
Spatial and temporal distribution of nerves, ganglia, and smooth muscle during the early pseudoglandular stage of fetal mouse lung development
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DOI：
10.1002/dvdy.1124
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发表时间：
2001-05-01
影响因子：
2.5
通讯作者：
Sparrow, MP
中科院分区：
文献类型：
--
作者：
Tollet, J;Everett, AW;Sparrow, MP
神经组织和平滑肌在胎儿肺发育早期出现，但对其起源和随后的分布知之甚少。为了研究早期假腺阶段的神经、神经节和气道平滑肌的时空分布，我们对胚胎日(E) 11至14的胎鼠肺进行了全载免疫染色和共聚焦显微镜成像。在E11，原始肺由未来的气管和两个正在萌芽的上皮小管组成，这些小管被平滑肌覆盖到生长芽的底部。进入肺的迷走神经和神经突中PGP 9.5（蛋白基因产物9.5）和突触蛋白阳性，但未见神经元染色。p75抗体（NTR）显示神经嵴细胞分布在未来气管、迷走神经和从迷走神经延伸到肺的突起中。这一发现表明，尽管在这一阶段神经元前体已经存在，但它们仍在向肺中迁移。到E12，肺近端神经组织丰富，神经沿着平滑肌覆盖的小管到达生长芽的底部。在E13和E14处，一个由迷走神经支配的相互连接的神经节神经网络覆盖了气管。节后神经主要沿平滑肌覆盖的小管分布，但也有部分神经延伸到上皮芽外的间质。此外，我们在体外培养的肺外植体模型中显示神经组织和平滑肌持续存在并继续生长和分化。通过荧光标记和共聚焦显微镜，我们将发育中的肺呈现为一个动态结构，平滑肌和神经组织处于影响生长发育的主要位置。(C) 2001 Wiley-Liss, Inc。
Neural tissue and smooth muscle appear early in the developing fetal lung, but little is known of their origin and subsequent distribution. To investigate the spatial and temporal distribution of nerves, ganglia, and airway smooth muscle during the early pseudoglandular stage, fetal mouse lungs at embryonic days (E) 11 to 14 were immunostained as whole-mounts and imaged by confocal microscopy, At E11, the primordial lung consisted of the future trachea and two budding epithelial tubules that were covered in smooth muscle to the base of the growing buds. The vagus and processes entering the lung were positive for the neural markers PGP 9.5 (protein gene product 9.5) and synapsin but no neurons were stained at this stage. An antibody to p75(NTR) revealed neural crest cells on the future trachea as well as in the vagus and in processes extending from the vagus to the lung. This finding indicates that even though neuronal precursors are already present at this stage, they are still migrating into the lung, By E12, neural tissue was abundant in the proximal part of the lung and nerves followed the smooth muscle-covered tubules to the base of the growing buds. At E13 and E14, a neural network of interconnected ganglia, innervated by the vagus, covered the trachea. The postganglionic nerves mainly followed the smooth muscle-covered tubules, but some extended out into the mesenchyme beyond the epithelial buds. Furthermore, we show in a model of cultured lung explants that neural tissue and smooth muscle persist and continue to grow and differentiate in vitro. By using fluorescent markers and confocal microscopy, we present the developing lung as a dynamic structure with smooth muscle and neural tissue in a prime position to influence growth and development. (C) 2001 Wiley-Liss, Inc.