Leaf yellowing and anthocyanin accumulation are two genetically independent strategies in response to nitrogen limitation in Arabidopsis thaliana
Leaf yellowing and anthocyanin accumulation are two genetically independent strategies in response to nitrogen limitation in Arabidopsis thaliana
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DOI：
10.1093/pcp/pci225
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发表时间：
2006-01-01
影响因子：
4.9
通讯作者：
Maselaux-Daubresse, U
中科院分区：
文献类型：
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作者：
Diaz, U;Saliba-Colombani, V;Maselaux-Daubresse, U
首次在拟南芥中，这项工作提出了与叶片衰老和胁迫反应症状，如黄化和花青素相关的红色相关的数量性状基因座（QTL）的鉴定。当拟南芥植物在低氮条件下培养时，我们观察到莲座丛的老叶变黄和整个莲座丛变红都得到促进。叶片黄化是与叶绿素分解有关的衰老症状。发红是与整个植物老化和营养限制有关的花青素苷积累的症状。在这项工作中，拟南芥被用作一个模式系统，剖析这些参数的遗传变异的QTL定位在415个重组自交系的海湾-0xShahdara人口。本研究共发现了15个新的QTL和2个上位性互作。通过目测法和图像处理法估计，莲座黄化分别受4个和5个QTL控制。通过对红度的目测，我们检测到6个QTL，其中主效QTL解释了总变异的33%。在近等基因系（异源自交系; HIF）中确认了两个主要的QTL，从而证实了这些性状的视觉符号的相关性。在以前的出版物中描述的叶片黄化，发红和氮利用效率的QTL之间的共定位表明复杂的相互关联的途径参与氮管理和衰老和应力相关的过程。未发现黄化和红度QTL间的共定位，表明这两个性状在遗传上是独立的。
For the first time in Arabidopsis thaliana, this work proposes the identification of quantitative trait loci (QTLs) associated with leaf senescence and stress response symptoms such as yellowing and anthocyanin-associated redness. When Arabidopsis plants were cultivated under low nitrogen conditions, we observed that both yellowing of the old leaves of the rosette and whole rosette redness were promoted. Leaf yellowing is a senescence symptom related to chlorophyll breakdown. Redness is a symptom of anthocyanin accumulation related to whole plant ageing and nutrient limitation. In this work, Arabidopsis is used as a model system to dissect the genetic variation of these parameters by QTL mapping in the 415 recombinant inbred lines of the Bay-0xShahdara population. Fifteen new QTLs and two epistatic interactions were described in this study. The yellowing of the rosette, estimated by visual notation and image processing, was controlled by four and five QTLs, respectively. The visual estimation of redness allowed us to detect six QTLs among which the major one explained 33%, of the total variation. Two main QTLs were confirmed in near-isogenic lines (heterogenous inbred family; HIF), thus confirming the relevance of the visual notation of these traits. Co-localizations between QTLs for leaf yellowing, redness and nitrogen use efficiency described in a previous publication indicate complex interconnected pathways involved in both nitrogen management and senescence- and stress-related processes. No co-localization between QTLs for leaf yellowing and redness has been found, suggesting that the two characters are genetically independent.