Digital holography of intracellular dynamics to probe tissue physiology.
Digital holography of intracellular dynamics to probe tissue physiology.
复制标题
DOI：
10.1364/ao.54.000a89
复制
发表时间：
2015-01-01
期刊：
影响因子：
1.9
通讯作者：
Nolte DD
中科院分区：
文献类型：
--
作者：
Merrill D;An R;Turek J;Nolte DD
数字全息技术提高了通过致密组织成像的能力。使用短相干光源，从背散射光记录的数字全息图执行激光测距，保持从深度获得的信息的保真度远远超过传统成像技术。生物动力成像技术（BDI）是一种发展中的活组织成像技术，深度可达一毫米，利用全息图强度波动作为无标签图像对比，可以研究原生微环境下的组织行为。本文采用BDI研究三维培养中粘连依赖性组织反应的变化。结果表明，细胞黏附密度的增加减缓了组织内的运动，并改变了对细胞骨架药物的反应。在中频（0.1 - 1 Hz）观察到膜波动的明显特征，这一特征因使用细胞松弛素d而增强，细胞松弛素d可降解细胞膜内的肌动蛋白皮质。这种增强特征仅在已经形成粘连的组织中观察到，因为细胞颗粒最初不表现出这种特征，但只有在孵育使粘连形成后才会发展出这种特征。
Digital holography provides improved capabilities for imaging through dense tissue. Using a short-coherence source, the digital hologram recorded from backscattered light performs laser ranging that maintains fidelity of information acquired from depths much greater than possible by traditional imaging techniques. Biodynamic Imaging (BDI) is a developing technology for live-tissue imaging of up to a millimeter in depth that uses the hologram intensity fluctuations as label-free image contrast and can study tissue behavior in native microenvironments. In this paper BDI is used investigate the change in adhesion-dependent tissue response in 3D cultures. The results show that increasing density of cellular adhesions slows motion inside tissue and alters the response to cytoskeletal drugs. A clear signature of membrane fluctuations was observed in mid frequencies (0.1 – 1 Hz) that was enhanced by the application of cytochalasin-D that degrades the actin cortex inside the cell membrane. This enhancement feature is only observed in tissues that have formed adhesions, because cell pellets initially do not show this signature, but develop this signature only after incubation enables adhesions to form.