The SCAR/WAVE complex polarizes PAN receptors and promotes division asymmetry in maize
The SCAR/WAVE complex polarizes PAN receptors and promotes division asymmetry in maize
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DOI：
10.1038/nplants.2014.24
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发表时间：
2015-01-26
期刊：
影响因子：
18
通讯作者：
Smith, Laurie G.
中科院分区：
文献类型：
--
作者：
Facette, Michelle R.;Park, Yeri;Smith, Laurie G.
有丝分裂前极性的建立是母细胞为不对称细胞分裂做准备的关键事件，不对称分裂产生不同命运的子代，并确保组织的正确细胞模式和最终的器官功能。先前的工作表明，两个受体样蛋白PANGLOSS2(PAN2)和PAN1以及植物的小GTP酶Rho GTP酶(ROP)促进了玉米气孔发育过程中母细胞的极性和随后的分裂不对称。PAN蛋白在细胞不对称分裂前发生极化，然而，这种极化的机制尚不清楚。在这里，我们证明了SCAR/WAVE调节复合体，它激活了肌动蛋白成核的Arp2/3复合体，是这个不对称分裂模型中第一个已知的极性标记，是PAN极化所必需的。这些发现表明肌动蛋白，特别是分支肌动蛋白网络，与PAN极化和不对称细胞分裂有关。
Pre-mitotic establishment of polarity is a key event in the preparation of mother cells for asymmetric cell divisions that produce daughters of distinct fates, and ensures correct cellular patterning of tissues and eventual organ function. Previous work has shown that two receptor-like kinases, PANGLOSS2 (PAN2) and PAN1, and the small GTPase RHO GTPASE OF PLANTS (ROP) promote mother cell polarity and subsequent division asymmetry in developing maize stomata. PAN proteins become polarized prior to asymmetric cell division, however, the mechanism of this polarization is unknown. Here we show that the SCAR/WAVE regulatory complex, which activates the actin-nucleating ARP2/3 complex, is the first known marker of polarity in this asymmetric division model and is required for PAN polarization. These findings implicate actin, and specifically branched actin networks, in PAN polarization and asymmetric cell division.