EM connectomics reveals axonal target variation in a sequence-generating network
EM connectomics reveals axonal target variation in a sequence-generating network
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DOI：
10.7554/elife.24364
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发表时间：
2017-03-27
期刊：
影响因子：
7.7
通讯作者：
Long, Michael A.
中科院分区：
文献类型：
--
作者：
Kornfeld, Joergen;Benezra, Sam E.;Long, Michael A.
在大脑的各个区域都观察到了神经元的连续激活，但在任何情况下，都没有很好地理解潜在的网络结构。在这里，我们研究了电路解剖斑马雀HVC，皮层区域，产生序列的时间进程的歌曲。我们结合连续块面电子显微镜与光学显微镜，以确定HVC（RA）神经元，控制歌曲的时间为目标的细胞类型。在它们的索马附近，轴突几乎专门针对抑制性中间神经元，这与从成对细胞的电记录中发现的一致。相反，远离索马的靶点主要是其他兴奋性神经元，其中约一半是其他HVC（RA）细胞。这两个观察结果都与这样的概念相一致，即调节歌曲节奏的神经序列是由嵌入局部抑制网络中的HVC中的全局突触链产生的。
The sequential activation of neurons has been observed in various areas of the brain, but in no case is the underlying network structure well understood. Here we examined the circuit anatomy of zebra finch HVC, a cortical region that generates sequences underlying the temporal progression of the song. We combined serial block-face electron microscopy with light microscopy to determine the cell types targeted by HVC(RA) neurons, which control song timing. Close to their soma, axons almost exclusively targeted inhibitory interneurons, consistent with what had been found with electrical recordings from pairs of cells. Conversely, far from the soma the targets were mostly other excitatory neurons, about half of these being other HVC(RA) cells. Both observations are consistent with the notion that the neural sequences that pace the song are generated by global synaptic chains in HVC embedded within local inhibitory networks.