In vivo 31P-NMR spectroscopy of right ventricle in pigs.
In vivo 31P-NMR spectroscopy of right ventricle in pigs.
复制标题
猪右心室体内 31P-NMR 波谱。
DOI：
10.1152/ajpheart.1992.262.6.h1950
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Matson,GB
中科院分区：
文献类型：
--
作者：
Schwartz,GG;Steinman,SK;Weiner,MW;Matson,GB
体内右心室（RV）的能量代谢在很大程度上尚未探索。本研究的目的是开发和实施RV游离壁的体内31 P核磁共振（NMR）光谱技术。构造了一个双匝、交叉设计的椭圆形表面线圈，以在薄的RV壁上提供高灵敏度，但在充满血液的RV腔中提供最小灵敏度。在36只开胸麻醉猪中，使用该线圈在2特斯拉的场强下进行了RV游离壁的31 P光谱分析。在24分钟内从800次采集中获得光谱，磷酸肌酸（PCr）的平均信噪比为13.2。猪RV的PCr与ATP（PCr/ATP）比值为1.42 +/- 0.05（平均值+/- SE），在1.8 s的重复时间下未校正饱和度。使用纵向松弛时间常数（T1）的文献值来校正部分饱和，估计RV PCr/ATP介于1.7和2.3之间。在RV缺血和压力超负荷期间观察到RV PCr/ATP降低。因此，在体内的RV的31 P光谱很容易完成与适当的表面线圈，可以提供新的信息RV能量代谢。
The energy metabolism of the right ventricle (RV) in vivo has been largely unexplored. The goal of this study was to develop and implement techniques for in vivo 31P nuclear magnetic resonance (NMR) spectroscopy of the RV free wall. A two-turn, crossover-design elliptical surface coil was constructed to provide high sensitivity across the thin RV wall but minimal sensitivity in the blood-filled RV cavity. In 36 open-chest, anesthetized pigs, 31P spectroscopy of the RV free wall was performed with this coil at a field strength of 2 Tesla. Spectra were obtained from 800 acquisitions in 24 min with an average signal-to-noise ratio of 13.2 for phosphocreatine (PCr). The PCr-to-ATP (PCr/ATP) ratio of porcine RV was 1.42 +/- 0.05 (mean +/- SE), uncorrected for saturation at a repetition time of 1.8 s. With the use of literature values of the time constant of longitudinal relaxation (T1) to correct for partial saturation, the RV PCr/ATP was estimated to lie between 1.7 and 2.3. Decreased RV PCr/ATP was observed during RV ischemia and pressure overload. Thus in vivo 31P spectroscopy of the RV is readily accomplished with an appropriate surface coil and can provide new information about RV energy metabolism.